NH2-Terminal Sequence of Human Factor V-Derived Fragments After HNE Digestion
| Cycle No. . | 99/97 kD* . | 89/87 kD . | 76/74 kD . |
|---|---|---|---|
| 1 | A(6.0) | V(7.0) | R(6.0) |
| 2 | Q(9.0) | G(8.0) | L(8.0) |
| 3 | L(10.0) | L(10.0) | L(9.0) |
| 4 | R(12.0) | S(5.0) | S(3.0) |
| 5 | Q(8.0) | K(6.0) | L(3.0) |
| 6 | F(10.0) | G(6.0) |
| Cycle No. . | 99/97 kD* . | 89/87 kD . | 76/74 kD . |
|---|---|---|---|
| 1 | A(6.0) | V(7.0) | R(6.0) |
| 2 | Q(9.0) | G(8.0) | L(8.0) |
| 3 | L(10.0) | L(10.0) | L(9.0) |
| 4 | R(12.0) | S(5.0) | S(3.0) |
| 5 | Q(8.0) | K(6.0) | L(3.0) |
| 6 | F(10.0) | G(6.0) |
Human F.V was incubated with 2 nmol/L HNE at 37°C for 2 and 30 minutes in HBS, pH 7.4 with 5 mmol/L CaCl2 as described in Materials and Methods. Approximately 900 pmol was used for each sample reaction. The apparent molecular mass of the specific fragment is shown along with the pmol of the amino acid identified (in parentheses) after each number of cycles performed.
Fragment doublets 99/97 kD; 89/87 kD; and 76/74 kD represent the HNE cleaved products of F.V.