Effects of Pre-incubation of HSCs or MS-5 With Oleic Acid or Linolenic Acid on HSC Proliferation
| Experiment 1 . | |||
|---|---|---|---|
| . | |||
| Linolenic Acid . | Pre-incubation of Low Density Cells . | Pre-incubation of MS-5 . | |
| . | 2 h . | 2 h . | 4 h . |
| 1 μg/mL | 0.39 ± 0.06 | 1.99 ± 0.305-150 | 2.22 ± 0.335-150 |
| Experiment 1 . | |||
|---|---|---|---|
| . | |||
| Linolenic Acid . | Pre-incubation of Low Density Cells . | Pre-incubation of MS-5 . | |
| . | 2 h . | 2 h . | 4 h . |
| 1 μg/mL | 0.39 ± 0.06 | 1.99 ± 0.305-150 | 2.22 ± 0.335-150 |
| Linolenic Acid . | Pre-incubation of P-HSCs . | Pre-incubation of MS-5 . |
|---|---|---|
| . | 2 h . | 2 h . |
| 0.5 μg/mL | ND | 2.36 ± 0.345-150 |
| 1 μg/mL | 0.86 ± 0.18 | 1.98 ± 0.305-150 |
| Linolenic Acid . | Pre-incubation of P-HSCs . | Pre-incubation of MS-5 . |
|---|---|---|
| . | 2 h . | 2 h . |
| 0.5 μg/mL | ND | 2.36 ± 0.345-150 |
| 1 μg/mL | 0.86 ± 0.18 | 1.98 ± 0.305-150 |
| Oleic Acid . | Pre-incubation of MS-5 . |
|---|---|
| . | 4 h . |
| 1 μg/mL | 1.86 ± 0.235-150 |
| 5 μg/mL | 2.33 ± 0.165-151 |
| 10 μg/mL | 2.56 ± 0.255-151- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - |
| 1 μg/mL | 2.44 ± 0.375-150 |
| 10 μg/mL | 1.76 ± 0.025-151 |
| Oleic Acid . | Pre-incubation of MS-5 . |
|---|---|
| . | 4 h . |
| 1 μg/mL | 1.86 ± 0.235-150 |
| 5 μg/mL | 2.33 ± 0.165-151 |
| 10 μg/mL | 2.56 ± 0.255-151- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - |
| 1 μg/mL | 2.44 ± 0.375-150 |
| 10 μg/mL | 1.76 ± 0.025-151 |
LD cells or MS-5 were pre-incubated with linolenic acid for 2 or 4 hours, then washed two times. These cells were cultured with nontreated MS-5 cells or with nontreated LD cells, respectively. The incorporation of 3H-thymidine was measured 5 days later (Exp 1). P-HSCs (instead of LD cells) were used in Exps 2 and 3. The incorporation of 3H-thymidine was assessed 10 days later. Stimulation indices: sample well/control well, in which nontreated LD cells or P-HSCs were cultured on nontreated MS-5 cells. Mean ± SE.
Different from control (P < .05).
P < .005.