Table 2. Stimulation Index of Various... | American Society of Hematology

Table 2.

Stimulation Index of Various Fractions Obtained From TJ-48 for HSC Proliferation

Experiment 1

	F-1-1	F-1-1
		B	D	E	F
0.1 μg/mL	2.09^*	1.17	1.28	1.80	0.87
0.5 μg/mL	1.96	0.90	1.11	1.95	1.50
1 μg/mL	1.77	1.10	1.16	2.71	1.68
2.5 μg/mL	1.55	0.89	0.14	0.22	0.32
5 μg/mL	1.38	ND	ND	ND	ND
10 μg/mL	1.46	ND	ND	ND	ND

Experiment 1

	F-1-1	F-1-1
		B	D	E	F
0.1 μg/mL	2.09^*	1.17	1.28	1.80	0.87
0.5 μg/mL	1.96	0.90	1.11	1.95	1.50
1 μg/mL	1.77	1.10	1.16	2.71	1.68
2.5 μg/mL	1.55	0.89	0.14	0.22	0.32
5 μg/mL	1.38	ND	ND	ND	ND
10 μg/mL	1.46	ND	ND	ND	ND

	F-1-1		E-4	E-5	E-6	E-7	WEHI-3 Cond. Med. (5%)^†
	E	F
0.1 μg/mL	ND	ND	1.01	1.21	1.17	0.97	1.60
0.5 μg/mL	ND	ND	1.09	1.43	1.28	1.06
1 μg/mL	1.98	1.42	1.30	1.49	1.95	1.68

	F-1-1		E-4	E-5	E-6	E-7	WEHI-3 Cond. Med. (5%)^†
	E	F
0.1 μg/mL	ND	ND	1.01	1.21	1.17	0.97	1.60
0.5 μg/mL	ND	ND	1.09	1.43	1.28	1.06
1 μg/mL	1.98	1.42	1.30	1.49	1.95	1.68

	E-5	E-5-1	E-5-2	E-5-3	E-5-4	E-5-5	WEHI-3 Cond. Med. (5%)
0.05 μg/mL	ND	1.41	1.16	1.51	1.37	1.48	1.22
0.1 μg/mL	0.97	1.60	1.45	1.56	1.67	1.41
0.2 μg/mL	ND	1.49	1.29	1.51	1.49	1.45
0.5 μg/mL	1.37	ND	ND	ND	ND	ND
1 μg/mL	1.40	ND	ND	ND	ND	ND

	E-5	E-5-1	E-5-2	E-5-3	E-5-4	E-5-5	WEHI-3 Cond. Med. (5%)
0.05 μg/mL	ND	1.41	1.16	1.51	1.37	1.48	1.22
0.1 μg/mL	0.97	1.60	1.45	1.56	1.67	1.41
0.2 μg/mL	ND	1.49	1.29	1.51	1.49	1.45
0.5 μg/mL	1.37	ND	ND	ND	ND	ND
1 μg/mL	1.40	ND	ND	ND	ND	ND

Abbreviation: ND, not determined.

Stimulation index was calculated using the following formula: Stimulation index = ³H-thymidine uptake on sample well/³H-thymidine uptake on control well. Values more than 1.3 are in boldface.

†

As a positive control, 5% of WEHI-3 conditioned medium was added to the culture medium instead of the sample.

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