Hyaluronic Acid and Chondroitin Sulfate A Binding of Vector-Only and CD44 Transfectants
| Transfectants . | No Addition . | cpm Bound . | Chondroitin Sulfate A . | (Fold Increase) . | |
|---|---|---|---|---|---|
| Hyaluronic Acid . | (Fold Increase) . | ||||
| Vector-only | |||||
| I | 33,564 ± 854 | 38,229 ± 2204 | (1.1×) | 39,352 ± 1399 | (1.2×) |
| II | 21,034 ± 699 | 32,161 ± 1550 | (1.5×) | 36,633 ± 912 | (1.7×) |
| CD44H | |||||
| I | 30,113 ± 938 | 65,419 ± 2418 | (2.2×) | 41,669 ± 1109 | (1.4×) |
| II | 22,525 ± 576 | 79,882 ± 889 | (3.5×) | 65,398 ± 2027 | (2.9×) |
| CD44ex7-14 | |||||
| I | 27,560 ± 1727 | 101,766 ± 2358 | (3.6×) | 85,804 ± 2537 | (3.1×) |
| II | 26,912 ± 1617 | 108,794 ± 6525 | (4.0×) | 50,506 ± 569 | (1.9×) |
| CD44ex10-14 | |||||
| I | 42,789 ± 872 | 136,912 ± 9765 | (3.2×) | 37,136 ± 755 | (0.9×) |
| II | 31,673 ± 958 | 91,457 ± 847 | (2.9×) | 39,096 ± 755 | (1.2×) |
| CD44ex10 | |||||
| I | 29,523 ± 2593 | 89,353 ± 427 | (3.0×) | 38,233 ± 1657 | (1.3×) |
| II | 40,586 ± 389 | 123,748 ± 5973 | (3.0×) | 51,581 ± 608 | (1.3×) |
| III | 43,009 ± 1242 | 124,258 ± 1713 | (2.8×) | 56,236 ± 3002 | (1.3×) |
| Transfectants . | No Addition . | cpm Bound . | Chondroitin Sulfate A . | (Fold Increase) . | |
|---|---|---|---|---|---|
| Hyaluronic Acid . | (Fold Increase) . | ||||
| Vector-only | |||||
| I | 33,564 ± 854 | 38,229 ± 2204 | (1.1×) | 39,352 ± 1399 | (1.2×) |
| II | 21,034 ± 699 | 32,161 ± 1550 | (1.5×) | 36,633 ± 912 | (1.7×) |
| CD44H | |||||
| I | 30,113 ± 938 | 65,419 ± 2418 | (2.2×) | 41,669 ± 1109 | (1.4×) |
| II | 22,525 ± 576 | 79,882 ± 889 | (3.5×) | 65,398 ± 2027 | (2.9×) |
| CD44ex7-14 | |||||
| I | 27,560 ± 1727 | 101,766 ± 2358 | (3.6×) | 85,804 ± 2537 | (3.1×) |
| II | 26,912 ± 1617 | 108,794 ± 6525 | (4.0×) | 50,506 ± 569 | (1.9×) |
| CD44ex10-14 | |||||
| I | 42,789 ± 872 | 136,912 ± 9765 | (3.2×) | 37,136 ± 755 | (0.9×) |
| II | 31,673 ± 958 | 91,457 ± 847 | (2.9×) | 39,096 ± 755 | (1.2×) |
| CD44ex10 | |||||
| I | 29,523 ± 2593 | 89,353 ± 427 | (3.0×) | 38,233 ± 1657 | (1.3×) |
| II | 40,586 ± 389 | 123,748 ± 5973 | (3.0×) | 51,581 ± 608 | (1.3×) |
| III | 43,009 ± 1242 | 124,258 ± 1713 | (2.8×) | 56,236 ± 3002 | (1.3×) |
Two or three independently derived vector-only, CD44H, CD44ex7-14, CD44ex10-14, and CD44ex10 transfectants were evaluated for adherence to immobilized hyaluronic acid, chondroitin sulfate A or plastic alone (no addition). Values are mean ± SE bound cpm for triplicate samples. The increase in hyaluronic acid or chondroitin sulfate A adhesion over background adhesion (no addition) for each transfectant is also shown (fold increase). Data are derived from one of three similar experiments.