Table 1. Relative Sensitivity of... | American Society of Hematology

Table 1.

Relative Sensitivity of Cytokines to DEX

Donor	Sex	TNF-α		IL-12(p40)		IL-10
Donor	Sex	ng/mL	IC50^-150	pg/mL	IC50^-150	pg/mL	IC50^-150
4	M	1.53	0.00001^-151	360	0.011	101	1.70^-151
5	M	1.38	0.021	98	0.0042	172	>100^-151
6	F	2.76	0.0051	696	0.0071	274	>100^-151
7	F	2.95	0.0052	617	0.0061	2,498	0.0015
8	F	0.60	0.012	382	0.31	235	>100^-151
9	M	5.15	0.080	308	0.082	218	>100^-151
10	M	1.29	0.015	143	0.021	394	0.022
11	F	1.24	0.081	226	0.082	382	0.33
12	F	3.95	0.020	772	0.033	343	0.20
13	M	ND	ND	945	0.021	106	4.0^-151
14	M	ND	ND	1,321	0.12	101	1.0
15	M	ND	ND	1,498	0.063	155	>100^-151
16	F	ND	ND	734	0.43	370	>100^-151
Mean IC50 ± SEM			0.026 ± 0.010		0.091 ± 0.036		1.036 ± 0.546^-152

Donor	Sex	TNF-α		IL-12(p40)		IL-10
Donor	Sex	ng/mL	IC50^-150	pg/mL	IC50^-150	pg/mL	IC50^-150
4	M	1.53	0.00001^-151	360	0.011	101	1.70^-151
5	M	1.38	0.021	98	0.0042	172	>100^-151
6	F	2.76	0.0051	696	0.0071	274	>100^-151
7	F	2.95	0.0052	617	0.0061	2,498	0.0015
8	F	0.60	0.012	382	0.31	235	>100^-151
9	M	5.15	0.080	308	0.082	218	>100^-151
10	M	1.29	0.015	143	0.021	394	0.022
11	F	1.24	0.081	226	0.082	382	0.33
12	F	3.95	0.020	772	0.033	343	0.20
13	M	ND	ND	945	0.021	106	4.0^-151
14	M	ND	ND	1,321	0.12	101	1.0
15	M	ND	ND	1,498	0.063	155	>100^-151
16	F	ND	ND	734	0.43	370	>100^-151
Mean IC50 ± SEM			0.026 ± 0.010		0.091 ± 0.036		1.036 ± 0.546^-152

Cytokine production in whole-blood cultures was induced with 250 ng/mL LPS. The supernatants were obtained after 24 hours of culture and the cytokines determined by ELISA.

Abbreviations: ND, not done; M, male; F, female.

F0-150

DEX concentration (μmol/L) required for 50% inhibition of cytokine production.

F0-151

Results obtained by extrapolation.

F0-152

N = 7, excluding values >100.

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