Effect of d-NMPs and ODNs on CD34+ Bone Marrow Cells
| d-NMP or ODN . | 3′ End . | Concentration (μmol/L) . | Cell Counts After 72 Hours . | |
|---|---|---|---|---|
| . | . | . | (% of Control ± SD) . | |
| . | . | . | 2.5% NHS . | 10% FCS . |
| d-AMP | 25 | 80 ± 2 | ||
| d-CMP | 25 | 106 ± 1 | ||
| d-GMP | 25 | 24 ± 6 | ||
| TMP | 25 | 53 ± 21 | ||
| d-NMP | 25 (100) | 92 ± 3 | ||
| PO1 | TAT | 25 | 42 ± 5 | |
| PO12 | TTC | 25 | 104 ± 3 | |
| PS1 | GAT | 10 | 38 ± 7 | |
| PS2 | GCG | 10 | 84 ± 5 | |
| PS3 | CTT | 10 | 30 ± 4 | |
| PS4 | GCG | 10 | 86 ± 11 | |
| PS5 | GCG | 10 | 83 ± 4 | |
| PS6 | GAT | 10 | 45 ± 1 | |
| d-NMP or ODN . | 3′ End . | Concentration (μmol/L) . | Cell Counts After 72 Hours . | |
|---|---|---|---|---|
| . | . | . | (% of Control ± SD) . | |
| . | . | . | 2.5% NHS . | 10% FCS . |
| d-AMP | 25 | 80 ± 2 | ||
| d-CMP | 25 | 106 ± 1 | ||
| d-GMP | 25 | 24 ± 6 | ||
| TMP | 25 | 53 ± 21 | ||
| d-NMP | 25 (100) | 92 ± 3 | ||
| PO1 | TAT | 25 | 42 ± 5 | |
| PO12 | TTC | 25 | 104 ± 3 | |
| PS1 | GAT | 10 | 38 ± 7 | |
| PS2 | GCG | 10 | 84 ± 5 | |
| PS3 | CTT | 10 | 30 ± 4 | |
| PS4 | GCG | 10 | 86 ± 11 | |
| PS5 | GCG | 10 | 83 ± 4 | |
| PS6 | GAT | 10 | 45 ± 1 | |
CD34+ bone marrow cell counts expressed as a percentage of control cells 72 hours after a single addition of d-NMP, d-NMP mixture or ODN, at concentrations from 25 to 100 μmol/L (for d-NMPs) or 10 to 25 μmol/L (for phosphorothioate or phosphodiester ODNs), in RPMI 1640 containing 2.5% NHS or 10% FCS. Data are expressed as the mean of three experiments (each on a different bone marrow) ± SD.