Effect of d-NMPs on BV173
| d-NMP . | Concentration (μmol/L) . | Cell Counts After 72 Hours . | ||
|---|---|---|---|---|
| . | . | (% of Control ± SD) . | ||
| . | . | 2.5% NHS . | 2.5% NHSΔ . | 10% FCS . |
| d-AMP | 1 | 108 ± 18 | ||
| d-AMP | 10 | 80 ± 7 | ||
| d-AMP | 25 | 76 ± 5 | 90 ± 10 | 79 ± 3 |
| d-AMP | 100 | 54 ± 4 | ||
| d-CMP | 1 | 106 ± 6 | ||
| d-CMP | 10 | 99 ± 13 | ||
| d-CMP | 25 | 114 ± 13 | 102 ± 18 | 99 ± 8 |
| d-CMP | 100 | 119 ± 23 | ||
| d-GMP | 1 | 105 ± 7 | ||
| d-GMP | 10 | 37 ± 15 | ||
| d-GMP | 25 | 29 ± 4 | 95 ± 8 | 37 ± 5 |
| d-GMP | 100 | 14 ± 2 | ||
| TMP | 1 | 107 ± 10 | ||
| TMP | 10 | 71 ± 7 | ||
| TMP | 25 | 50 ± 1 | 97 ± 5 | 61 ± 13 |
| TMP | 100 | 41 ± 8 | ||
| d-NMPs | 100 (400) | 94 ± 5 | ||
| d-AMP+d-CMP | 25 (50) | 113 ± 15 | ||
| d-AMP+d-GMP | 25 (50) | 41 ± 11 | ||
| d-AMP+TMP | 25 (50) | 45 ± 7 | ||
| d-CMP+d-GMP | 25 (50) | 59 ± 9 | ||
| d-CMP+TMP | 25 (50) | 113 ± 21 | ||
| d-GMP+TMP | 25 (50) | 17 ± 5 | ||
| d-NMP . | Concentration (μmol/L) . | Cell Counts After 72 Hours . | ||
|---|---|---|---|---|
| . | . | (% of Control ± SD) . | ||
| . | . | 2.5% NHS . | 2.5% NHSΔ . | 10% FCS . |
| d-AMP | 1 | 108 ± 18 | ||
| d-AMP | 10 | 80 ± 7 | ||
| d-AMP | 25 | 76 ± 5 | 90 ± 10 | 79 ± 3 |
| d-AMP | 100 | 54 ± 4 | ||
| d-CMP | 1 | 106 ± 6 | ||
| d-CMP | 10 | 99 ± 13 | ||
| d-CMP | 25 | 114 ± 13 | 102 ± 18 | 99 ± 8 |
| d-CMP | 100 | 119 ± 23 | ||
| d-GMP | 1 | 105 ± 7 | ||
| d-GMP | 10 | 37 ± 15 | ||
| d-GMP | 25 | 29 ± 4 | 95 ± 8 | 37 ± 5 |
| d-GMP | 100 | 14 ± 2 | ||
| TMP | 1 | 107 ± 10 | ||
| TMP | 10 | 71 ± 7 | ||
| TMP | 25 | 50 ± 1 | 97 ± 5 | 61 ± 13 |
| TMP | 100 | 41 ± 8 | ||
| d-NMPs | 100 (400) | 94 ± 5 | ||
| d-AMP+d-CMP | 25 (50) | 113 ± 15 | ||
| d-AMP+d-GMP | 25 (50) | 41 ± 11 | ||
| d-AMP+TMP | 25 (50) | 45 ± 7 | ||
| d-CMP+d-GMP | 25 (50) | 59 ± 9 | ||
| d-CMP+TMP | 25 (50) | 113 ± 21 | ||
| d-GMP+TMP | 25 (50) | 17 ± 5 | ||
BV173 viable cell counts (expressed as a percentage of control cell counts) 72 hours after a single addition of d-NMP (or of d-NMP mixture), at concentrations from 1 to 400 μmol/L in RPMI 1640 containing 2.5% NHS, 2.5% NHS heat-treated at 60°C for 30 minutes (NHSΔ), or 10% FCS. Data are expressed as the mean of at least three experiments ± SD. The d-NMP mixtures are composed of equimolar amounts of each component. The total d-NMP concentration is indicated between brackets.