Table 1. Adhesion of Erythroid... | American Society of Hematology

Table 1.

Adhesion of Erythroid Progenitors to Extracellular Matrix Proteins

Substrate	% Adhesion
	CFU-E	Mature BFU-E	Primitive BFU-E	HEPA1
BSA	6 ± 1	<1	<1	<5
	n = 19	n = 21	n = 21	n = 3
Fibronectin	76 ± 2^*	5 ± 2^*	11 ± 2^*	83 ± 5^*
	n = 19	n = 13	n = 16	n = 3
Laminin	16 ± 1^*	12 ± 2^*	12 ± 4^*	69 ± 4^*
	n = 4	n = 7	n = 7	n = 3
Collagen types I and III	13 ± 3	<5	<1	78 ± 7^*
	n = 4	n = 2	n = 2	n = 3
Collagen type IV	15 ± 2^*	13 ± 3	<5	82 ± 3^*
	n = 7	n = 2	n = 2	n = 3

Substrate	% Adhesion
	CFU-E	Mature BFU-E	Primitive BFU-E	HEPA1
BSA	6 ± 1	<1	<1	<5
	n = 19	n = 21	n = 21	n = 3
Fibronectin	76 ± 2^*	5 ± 2^*	11 ± 2^*	83 ± 5^*
	n = 19	n = 13	n = 16	n = 3
Laminin	16 ± 1^*	12 ± 2^*	12 ± 4^*	69 ± 4^*
	n = 4	n = 7	n = 7	n = 3
Collagen types I and III	13 ± 3	<5	<1	78 ± 7^*
	n = 4	n = 2	n = 2	n = 3
Collagen type IV	15 ± 2^*	13 ± 3	<5	82 ± 3^*
	n = 7	n = 2	n = 2	n = 3

Bone marrow cells were incubated in wells coated with the indicated substrates. The unattached and attached populations were then assayed for the presence of CFU-Es, mature BFU-Es, and primitive BFU-Es. Hepal cell adhesion assays were performed as previously described (Patel et al39 ). Briefly, the cells were metabollically labeled and incubated in wells coated with the indicated substrates. The ratio of adherent cpm to the input cpm was then calculated. All results are expressed as the mean percent cell adhesion ± SEM.

P ≤ .02.

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