Regulation of Human CD34+ Cell Growth From PB by Cytokines in Expansion Culture
| . | KL . | IL-1 . | IL-3 . | K+F . | K+6 . | K+6+s6R . | 1+3 . | K+1 . | K+3 . | K36 . | K36EG . | K36EG + . | K36EG + TGF-β1 100 . | F36EG . | KF36EG . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | . | . | . | . | . | . | TGF-β1 10 . | . | . | . |
| Day 3 | 0.5 | 0.5 | 0.5 | 0.6 | 0.3 | - | 0.6 | 0.5 | 0.5 | 0.8 | 3.0 | 0.7 | 0.2 | 0.8 | 3.2 |
| Day 5 | 0.5 | 0.7 | 0.7 | 0.9 | 0.6 | - | 0.9 | 0.9 | 1.0 | 1.0 | 8.6 | 1.0 | 0.9 | 5.2 | 10.0 |
| Day 7* | 1.2 | 1.4 | 2.0 | 1.5 | 1.7 | 2.1 | 2.2 | 2.3 | 3.5 | 5.0 | 24.4 | 2.6 | 2.0 | 12.0 | 35.0 |
| . | KL . | IL-1 . | IL-3 . | K+F . | K+6 . | K+6+s6R . | 1+3 . | K+1 . | K+3 . | K36 . | K36EG . | K36EG + . | K36EG + TGF-β1 100 . | F36EG . | KF36EG . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | . | . | . | . | . | . | TGF-β1 10 . | . | . | . |
| Day 3 | 0.5 | 0.5 | 0.5 | 0.6 | 0.3 | - | 0.6 | 0.5 | 0.5 | 0.8 | 3.0 | 0.7 | 0.2 | 0.8 | 3.2 |
| Day 5 | 0.5 | 0.7 | 0.7 | 0.9 | 0.6 | - | 0.9 | 0.9 | 1.0 | 1.0 | 8.6 | 1.0 | 0.9 | 5.2 | 10.0 |
| Day 7* | 1.2 | 1.4 | 2.0 | 1.5 | 1.7 | 2.1 | 2.2 | 2.3 | 3.5 | 5.0 | 24.4 | 2.6 | 2.0 | 12.0 | 35.0 |
Abbreviations: Kit-ligand (KL), K; Flk-2 ligand, F; IL-1, 1; IL-3, 3; IL-6, 6; Erythropoietin, E; G-CSF, G; sIL-6R, s6R.
Cell number increase = number of cells/input cell number in Delta.