Percentage of Phase II and Phase III Plus IV Cells Residing in G0/G1 After 14 Days of Sequential Cytokine Stimulation
| Phase . | Percentage of Cells in G0/G*1 . | ||||
|---|---|---|---|---|---|
| . | Cytokine Treatment: Day 0-7/Day 7-14 . | ||||
| . | SCF/SCF . | IL-3/FL . | IL-3/SCF . | FL/IL-3 . | SCF/IL-3 + SCF . |
| II | 92 | 93 | 92 | 88 | 81 |
| III and IV | 92 | 86 | 84 | 69 | 76 |
| Phase . | Percentage of Cells in G0/G*1 . | ||||
|---|---|---|---|---|---|
| . | Cytokine Treatment: Day 0-7/Day 7-14 . | ||||
| . | SCF/SCF . | IL-3/FL . | IL-3/SCF . | FL/IL-3 . | SCF/IL-3 + SCF . |
| II | 92 | 93 | 92 | 88 | 81 |
| III and IV | 92 | 86 | 84 | 69 | 76 |
Sorted G0 CD34+ cells were cultured under sequential cytokine stimulation as indicated for a period of 14 days. Phase II and phase III plus IV cells were sorted on day 14, stained with propidium iodide, and analyzed for cell cycle status. Results shown were derived from one representative experiment out of three. Similar results were obtained in the other two experiments. All experiments contained the same cytokine combinations shown in the first four columns.