Time-Dependent Effects of Dex and/or IL-6 on Cell Cycle Distribution of OCI-My5 Cells
| . | Time (h) . | |||||||
|---|---|---|---|---|---|---|---|---|
| . | 0 . | 2 . | 4 . | 8 . | 16 . | 24 . | 36 . | 48 . |
| Media | 40.5 | 40.7 | 41 | 41.3 | 41.5 | 42.7 | 43 | 43.1 |
| IL-6 (50 ng/mL) | 40.5 | 40.8 | 39.2 | 38.8 | 31.8 | 31 | 30.2 | 31.4 |
| Dex (1 μmol/L) | 40.5 | 40.7 | 45.7 | 55.7 | 62.5 | 62.6 | 62.2 | 62.8 |
| IL-6 (50 ng/mL) + Dex (1 μmol/L) | 40.5 | 41.6 | 40.9 | 39.3 | 34.6 | 33.7 | 33.5 | 32.3 |
| . | Time (h) . | |||||||
|---|---|---|---|---|---|---|---|---|
| . | 0 . | 2 . | 4 . | 8 . | 16 . | 24 . | 36 . | 48 . |
| Media | 40.5 | 40.7 | 41 | 41.3 | 41.5 | 42.7 | 43 | 43.1 |
| IL-6 (50 ng/mL) | 40.5 | 40.8 | 39.2 | 38.8 | 31.8 | 31 | 30.2 | 31.4 |
| Dex (1 μmol/L) | 40.5 | 40.7 | 45.7 | 55.7 | 62.5 | 62.6 | 62.2 | 62.8 |
| IL-6 (50 ng/mL) + Dex (1 μmol/L) | 40.5 | 41.6 | 40.9 | 39.3 | 34.6 | 33.7 | 33.5 | 32.3 |
OCI-My5 cells (5 × 105) were cultured with 10% FBS Iscove's media alone, IL-6, Dex, or IL-6 + Dex. Cells (1 × 106) were harvested at intervals, washed, stained with PI, and analyzed using FACS. Values are the percentage of cells in G1 phase.