Inhibitory Effects of MIP-1α on BFU-E Formation
| Growth Factor . | Frequency of BFU-E Formation . |
|---|---|
| EPO | 1:288 |
| EPO + MIP-1α | 0:288 |
| EPO + SCF | 1:19.2 |
| EPO + SCF + MIP-1α | 1:57.6 |
| EPO + SCF + MIP-1α + anti-CCR1 | 1:243-150 |
| EPO + SCF + MIP-1α + anti-GST | 1:57.6 |
| Growth Factor . | Frequency of BFU-E Formation . |
|---|---|
| EPO | 1:288 |
| EPO + MIP-1α | 0:288 |
| EPO + SCF | 1:19.2 |
| EPO + SCF + MIP-1α | 1:57.6 |
| EPO + SCF + MIP-1α + anti-CCR1 | 1:243-150 |
| EPO + SCF + MIP-1α + anti-GST | 1:57.6 |
Inhibitory effects of MIP-1α on BFU-E formation from CD34+ cells was partially reversed by anti-CCR1 antibodies. CD34+ cells were seeded at a concentration of 1 cell per well into a well in three 96-well plates in a methylcellulose medium containing different combinations of Epo, SCF, recombinant human MIP-1α, anti-CCR1 or control antibodies as indicated. The number of BFU-E was determined at day 14 after the culture. The data are representative of three independent experiments.
Significant difference from control culture without anti-CCR1 antibody by using one-way ANOVA analysis and multiple comparison by Fisher's method.