Frequencies of Donor-Reactive T-Cells in the Lethally Irradiated, Allogeneic Host After Transfer of BMC From rCD44v4-v7-TG and NTG Mice
| Organ . | Days After Reconstitution5-150 . | Donor-Reactive T Cells†: Frequency/106 Cells . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| . | . | Transfer: NTG BMC . | Transfer: TG BMC . | . | . | . | |||
| . | . | Unseparated . | Without T and B . | Without T and B + rCD44v6+ BMC . | Unseparated . | Without rCD44v6+ BMC . | . | . | . |
| BMC | 14 | ND | ND | ND [NS] | ND [NS] | ND [NS] | |||
| 21 | 93 (296) | 72 (212) | 120 [NS] (375) | 127 [NS] (358) | 56 [NS] (169) | ||||
| 28 | 424 (2,507) | 228 (1,562) | 500 [NS] (3,571) | 367 [NS] (1,782) | 255 [NS] (1,417) | ||||
| TC | 14 | 9 (26) | 7 (13) | 7 [NS] (16) | 10 [NS] (31) | 7 [NS] (14) | |||
| 21 | 37 (94) | 32 (97) | 34 [NS] (112) | 21 [NS] (121) | 13 [NS] (87) | ||||
| 28 | 37 (106) | 32 (91) | 15 [NS] (65) | 31 [NS] (265) | 17 [NS] (148) | ||||
| 42 | 63 (745) | 65 (619) | NT | 65-152 (85) | 4 [NS] (67) | ||||
| SC | 14 | ND | ND | ND [NS] | ND [NS] | ND [NS] | |||
| 21 | 149 (283) | 138 (317) | 164 [NS] (410) | 465-152 (158) | 50 [NS] (172) | ||||
| 28 | 1,852 (6,212) | 2,000 (6,667) | 833 [NS] (5,950) | 2865-152 (1,652) | 222 [NS] (1,586) | ||||
| 42 | 50 (345) | 81 (1,013) | 74 [NS] (1,088) | 85-152 (88) | 16 [NS] (134) | ||||
| LNC | 14 | ND | ND | ND [NS] | ND [NS] | ND [NS] | |||
| 21 | ND | ND | ND [NS] | ND [NS] | ND [NS] | ||||
| 28 | 167 (601) | 200 (500) | 111 [NS] (333) | 285-152 (116) | 31 [NS] (124) | ||||
| Organ . | Days After Reconstitution5-150 . | Donor-Reactive T Cells†: Frequency/106 Cells . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| . | . | Transfer: NTG BMC . | Transfer: TG BMC . | . | . | . | |||
| . | . | Unseparated . | Without T and B . | Without T and B + rCD44v6+ BMC . | Unseparated . | Without rCD44v6+ BMC . | . | . | . |
| BMC | 14 | ND | ND | ND [NS] | ND [NS] | ND [NS] | |||
| 21 | 93 (296) | 72 (212) | 120 [NS] (375) | 127 [NS] (358) | 56 [NS] (169) | ||||
| 28 | 424 (2,507) | 228 (1,562) | 500 [NS] (3,571) | 367 [NS] (1,782) | 255 [NS] (1,417) | ||||
| TC | 14 | 9 (26) | 7 (13) | 7 [NS] (16) | 10 [NS] (31) | 7 [NS] (14) | |||
| 21 | 37 (94) | 32 (97) | 34 [NS] (112) | 21 [NS] (121) | 13 [NS] (87) | ||||
| 28 | 37 (106) | 32 (91) | 15 [NS] (65) | 31 [NS] (265) | 17 [NS] (148) | ||||
| 42 | 63 (745) | 65 (619) | NT | 65-152 (85) | 4 [NS] (67) | ||||
| SC | 14 | ND | ND | ND [NS] | ND [NS] | ND [NS] | |||
| 21 | 149 (283) | 138 (317) | 164 [NS] (410) | 465-152 (158) | 50 [NS] (172) | ||||
| 28 | 1,852 (6,212) | 2,000 (6,667) | 833 [NS] (5,950) | 2865-152 (1,652) | 222 [NS] (1,586) | ||||
| 42 | 50 (345) | 81 (1,013) | 74 [NS] (1,088) | 85-152 (88) | 16 [NS] (134) | ||||
| LNC | 14 | ND | ND | ND [NS] | ND [NS] | ND [NS] | |||
| 21 | ND | ND | ND [NS] | ND [NS] | ND [NS] | ||||
| 28 | 167 (601) | 200 (500) | 111 [NS] (333) | 285-152 (116) | 31 [NS] (124) | ||||
The compared populations (NTG BMC v TG BMC; NTG BMC without T and B cells v NTG BMC without T and B cells + CD44v6+ TG BMC; and TG BMC v TG BMC without CD44v6+ cells) are indicated by the horizontal brackets.
Abbreviations: NS, nonsignificant; ND, not detectable; NT, not tested.
5 × 106 BMC, IV; where indicated, BMC were depleted of B and T cells or of rCD44v6+ cells as described in the Materials and Methods or BMC from NTG mice were depleted of B and T cells and substituted with rCD44v6+ BMC from TG mice (NTG BMC:CD44v6+ TG BMC = 9:1).
In parentheses is the frequency/106 cells corrected for the relative percentage of host-derived lymphocytes.
P ≤ .01.