Frequencies of Host-Reactive T Cells in the Lethally Irradiated, Allogeneic Host After Transfer of BMC From rCD44v4-v7-TG and NTG Mice
| . | . | Host-Reactive T Cells†: Frequency/106 Cells . | ||||
|---|---|---|---|---|---|---|
| . | . | . | . | . | . | . |
| Organ . | Days After Reconstitution4-150 . | Transfer: BMC . | Transfer: BMC Without T and B . | Transfer: BMC Without T and B NTG Donor + TG T Cells . | ||
| . | . | NTG Donor . | TG Donor . | NTG Donor . | TG Donor . | . |
| BMC | 14 | 323 (564) | 333 [NS] (564) | 294 (510) | 323 [NS] (514) | 345 (48) |
| 21 | 1,084 (1,608) | 244‡ (364) | 1,350 (2,015) | 214‡ (332) | 1,280 (1,988) | |
| 28 | 448 (575) | 24‡ (319) | 454 (593) | 29‡ (39) | 440 (598) | |
| 42 | 17 (20) | 6‡ (7) | 16 (19) | 5‡ (6) | 19 (23) | |
| TC | 14 | 2 (8) | 15‡ (26) | 2 (7) | ND [NS] | ND |
| 21 | 302 (667) | 2,500‡ (4,082) | 240 (545) | 1,389‡ (2,130) | 320 (727) | |
| 28 | 743 (1,236) | 42‡ (61) | 400 (565) | 12‡ (18) | 370 (546) | |
| 42 | 20 (26) | 2‡ (3) | 15 (17) | 4‡ (5) | 29 (33) | |
| SC | 14 | 135 (365) | 345 [NS] (759) | 102 (304) | 22‡ (52) | 114 (374) |
| 21 | 1,087 (2,558) | 738 [NS] (1,175) | 1,250 (3,605) | 320‡ (451) | 1,043 (2,249) | |
| 28 | 565 (1,101) | 137‡ (203) | 555 (914) | 112‡ (156) | 355 (544) | |
| 42 | 99 (151) | 14‡ (18) | 64 (82) | 15‡ (20) | 53 (72) | |
| LNC | 14 | ND | 22‡ (40) | ND | 5 [NS] (10) | ND |
| 21 | 4,900 (1,225) | 5,000 [NS] (8,197) | 4,500 (10,638) | 2,000‡ (3,724) | 4,700 (13,277) | |
| 28 | 645 (1,187) | 122‡ (175) | 480 (714) | 143‡ (202) | 457 (774) | |
| 42 | 80 (123) | 10‡ (12) | 75 (114) | ND‡ | 78 (127) | |
| . | . | Host-Reactive T Cells†: Frequency/106 Cells . | ||||
|---|---|---|---|---|---|---|
| . | . | . | . | . | . | . |
| Organ . | Days After Reconstitution4-150 . | Transfer: BMC . | Transfer: BMC Without T and B . | Transfer: BMC Without T and B NTG Donor + TG T Cells . | ||
| . | . | NTG Donor . | TG Donor . | NTG Donor . | TG Donor . | . |
| BMC | 14 | 323 (564) | 333 [NS] (564) | 294 (510) | 323 [NS] (514) | 345 (48) |
| 21 | 1,084 (1,608) | 244‡ (364) | 1,350 (2,015) | 214‡ (332) | 1,280 (1,988) | |
| 28 | 448 (575) | 24‡ (319) | 454 (593) | 29‡ (39) | 440 (598) | |
| 42 | 17 (20) | 6‡ (7) | 16 (19) | 5‡ (6) | 19 (23) | |
| TC | 14 | 2 (8) | 15‡ (26) | 2 (7) | ND [NS] | ND |
| 21 | 302 (667) | 2,500‡ (4,082) | 240 (545) | 1,389‡ (2,130) | 320 (727) | |
| 28 | 743 (1,236) | 42‡ (61) | 400 (565) | 12‡ (18) | 370 (546) | |
| 42 | 20 (26) | 2‡ (3) | 15 (17) | 4‡ (5) | 29 (33) | |
| SC | 14 | 135 (365) | 345 [NS] (759) | 102 (304) | 22‡ (52) | 114 (374) |
| 21 | 1,087 (2,558) | 738 [NS] (1,175) | 1,250 (3,605) | 320‡ (451) | 1,043 (2,249) | |
| 28 | 565 (1,101) | 137‡ (203) | 555 (914) | 112‡ (156) | 355 (544) | |
| 42 | 99 (151) | 14‡ (18) | 64 (82) | 15‡ (20) | 53 (72) | |
| LNC | 14 | ND | 22‡ (40) | ND | 5 [NS] (10) | ND |
| 21 | 4,900 (1,225) | 5,000 [NS] (8,197) | 4,500 (10,638) | 2,000‡ (3,724) | 4,700 (13,277) | |
| 28 | 645 (1,187) | 122‡ (175) | 480 (714) | 143‡ (202) | 457 (774) | |
| 42 | 80 (123) | 10‡ (12) | 75 (114) | ND‡ | 78 (127) | |
Abbreviations: NS, nonsignificant; ND, not detectable.
5 × 106 BMC, IV; where indicated, BMC were depleted of B and T cells as described in the Materials and Methods or BMC from NTG mice were depleted of B and T cells and substituted with mature T cells from rCD44v4-v7 TG mice (BMC:T cells = 9:1).
In parentheses is the frequency/106 cells corrected for the relative percentage of donor-derived lymphocytes.
P ≤ .01.