Effect of Nucleoside Analogs on the Incorporation of [3H]BrdUrd and [3H]dThd
| Analog . | Dose (nmol/L) . | % of Control (mean ± SD) . | ||
|---|---|---|---|---|
| . | . | [3H]BrdUrd . | [3H]dThd . | [3H]dThd ± . |
| . | . | . | . | Cold BrdUrd . |
| ara-C | 60 | 27.3 ± 3.7 | 93.2 ± 7.4 | 24.3 ± 4.1 |
| 6 | 72.7 ± 5.6 | 104.6 ± 6.8 | 68.1 ± 2.1 | |
| 0.6 | 102.2 ± 3.8 | 107.7 ± 7.2 | 102.5 ± 8.7 | |
| dFdC | 80 | 26.0 ± 4.5 | 55.7 ± 4.6 | 29.9 ± 2.8 |
| 8 | 87.3 ± 3.7 | 101.6 ± 6.3 | 95.3 ± 4.1 | |
| 0.8 | 107.3 ± 4.9 | 102.1 ± 8.4 | 100.3 ± 8.2 | |
| F-ara-A | 120 | 98.6 ± 6.4 | 103.8 ± 7.2 | 100.2 ± 8.9 |
| Analog . | Dose (nmol/L) . | % of Control (mean ± SD) . | ||
|---|---|---|---|---|
| . | . | [3H]BrdUrd . | [3H]dThd . | [3H]dThd ± . |
| . | . | . | . | Cold BrdUrd . |
| ara-C | 60 | 27.3 ± 3.7 | 93.2 ± 7.4 | 24.3 ± 4.1 |
| 6 | 72.7 ± 5.6 | 104.6 ± 6.8 | 68.1 ± 2.1 | |
| 0.6 | 102.2 ± 3.8 | 107.7 ± 7.2 | 102.5 ± 8.7 | |
| dFdC | 80 | 26.0 ± 4.5 | 55.7 ± 4.6 | 29.9 ± 2.8 |
| 8 | 87.3 ± 3.7 | 101.6 ± 6.3 | 95.3 ± 4.1 | |
| 0.8 | 107.3 ± 4.9 | 102.1 ± 8.4 | 100.3 ± 8.2 | |
| F-ara-A | 120 | 98.6 ± 6.4 | 103.8 ± 7.2 | 100.2 ± 8.9 |
CCRF-CEM cells were treated with nucleoside analogs at various concentrations and simultaneously incubated with 1.0 μmol/L [3H]BrdUrd (0.02 μCi/mL), [3H]dThd (0.2 μCi/mL), or [3H]dThd (0.2 μCi/mL) combined with unlabeled 1.0 μmol/L BrdUrd for 60 minutes. The results are triplicate determinations. Radioactivity was incorporated into DNA in control cells not treated with nucleotide analogs as follows: [3H]BrdUrd, 3,355 ± 155 dpm/106 cells; [3H]dThd, 202,008 ± 22,304 dpm/106; [3H]dThd combined with nonradioactive BrdUrd (1.0 μmol/L), 16,619 ± 789 dpm/106 cells.