Differential Blood Cell Counts
| Phenotype . | Group . | Weeks Posttransplantation . | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 9 . | 10 . | 11 . | 12 . | 13 . |
| Neutrophil (×10−3/μL) | Control | 4.1 | 0.9 | 0.8 | 1.2 | 1.2 | 1.0 | 1.1 | 1.2 | 1.0 | 1.1 | 1.0 | 0.9 | 1.1 |
| (0.54) | (0.07) | (0.08) | (0.06) | (0.05) | (0.15) | (0.1) | (0.07) | (0.14) | (0.09) | (0.06) | (0.17) | (0.09) | ||
| FL | 2.3 | 2.5* | 3.8* | 18.9* | 27.8* | 11.7 | 10.0 | 7.6 | 14.2 | 20.4 | 21.3 | 21.1 | 22.8 | |
| (0.28) | (0.20) | (0.56) | (3.38) | (3.33) | (3.17) | (2.26) | (1.64) | (4.26) | (7.60) | (8.27) | (17.0) | (17.0) | ||
| Lymphocyte (×10−3/μL) | Control | 1.5 | 1.9 | 3.4 | 6.3 | 6.3 | 9.3 | 7.2 | 10.1 | 12.3 | 11.6 | 9.8 | 7.3 | 12.3 |
| (0.14) | (0.14) | (0.16) | (0.32) | (0.52) | (1.0) | (0.61) | (1.05) | (1.06) | (1.02) | (1.13) | (1.54) | (0.87) | ||
| FL | 0.9 | 4.9* | 5.4* | 10.8† | 21.5* | 18.5 | 12.9 | 14.5 | 26.0 | 29.1 | 29.0† | 25.9 | 23.7 | |
| (0.08) | (0.45) | (0.41) | (1.15) | (2.08) | (4.26) | (2.74) | (3.06) | (3.26) | (4.77) | (2.47) | (7.1) | (7.28) | ||
| Monocyte (×10−3/μL) | Control | 0.5 | 0.2 | 0.2 | 0.3 | 0.2 | 0.3 | 0.2 | 0.3 | 0.4 | 0.3 | 0.3 | 0.3 | 0.5 |
| (0.07) | (0.03) | (0.01) | (0.02) | (0.01) | (0.03) | (0.02) | (0.02) | (0.04) | (0.04) | (0.04) | (0.11) | (0.11) | ||
| FL | 0.2 | 0.7* | 0.8* | 2.4* | 4.2* | 7.7 | 1.7† | 2.6† | 4.7† | 6.9 | 5.6 | 4.2 | 3.1 | |
| (0.02) | (0.08) | (0.06) | (0.22) | (0.42) | (5.45) | (0.33) | (0.46) | (0.9) | (1.93) | (1.23) | (2.35) | (1.66) | ||
| Eosinophil (×10−3/μL) | Control | 0.02 | 0.38 | 0.12 | 0.16 | 0.17 | 0.31 | 0.25 | 0.23 | 0.27 | 0.22 | 0.27 | 0.15 | 0.36 |
| (0.002) | (0.1) | (0.01) | (0.02) | (0.03) | (0.08) | (0.06) | (0.03) | (0.03) | (0.04) | (0.04) | (0.04) | (0.02) | ||
| FL | 0.01 | 0.13 | 0.40 | 0.33 | 0.22 | 0.10 | 0.12 | 0.42 | 0.45 | 0.42 | 0.40 | 0.18 | 0.12 | |
| (0.002) | (0.03) | (0.04) | (0.04) | (0.04) | (0.04) | (0.03) | (0.09) | (0.10) | (0.04) | (0.02) | (0.04) | (0.03) | ||
| Basophil (×10−3/μL) | Control | 0.02 | 0.01 | 0.03 | 0.04 | 0.03 | 0.04 | 0.04 | 0.05 | 0.11 | 0.06 | 0.09 | 0.03 | 0.05 |
| (0.004) | (0.002) | (0.003) | (0.01) | (0.003) | (0.01) | (0.01) | (0.01) | (0.03) | (0.02) | (0.02) | (0.01) | (0.01) | ||
| FL | 0.01 | 0.06* | 0.08* | 0.28* | 0.54* | 0.28† | 0.17 | 0.30 | 0.79 | 0.70 | 0.61 | 0.53 | 0.44 | |
| (0.002) | (0.01) | (0.01) | (0.04) | (0.09) | (0.06) | (0.05) | (0.1) | (0.31) | (0.29) | (0.17) | (0.38) | (0.32) | ||
| LUCs (×10−3/μL) | Control | 0.1 | 0.03 | 0.03 | 0.09 | 0.08 | 0.04 | 0.07 | 0.13 | 0.15 | 0.15 | 0.13 | 0.04 | 0.23 |
| (0.01) | (0.004) | (0.004) | (0.02) | (0.01) | (0.02) | (0.01) | (0.02) | (0.03) | (0.02) | (0.03) | (0.01) | (0.12) | ||
| FL | 0.06 | 0.27† | 0.27* | 0.77* | 2.06* | 0.15 | 0.86 | 1.07 | 1.14 | 2.38 | 1.80 | 1.01 | 2.05 | |
| (0.01) | (0.06) | (0.04) | (0.12) | (0.24) | (0.03) | (0.28) | (0.35) | (0.29) | (0.97) | (0.63) | (0.73) | (1.67) | ||
| Phenotype . | Group . | Weeks Posttransplantation . | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 9 . | 10 . | 11 . | 12 . | 13 . |
| Neutrophil (×10−3/μL) | Control | 4.1 | 0.9 | 0.8 | 1.2 | 1.2 | 1.0 | 1.1 | 1.2 | 1.0 | 1.1 | 1.0 | 0.9 | 1.1 |
| (0.54) | (0.07) | (0.08) | (0.06) | (0.05) | (0.15) | (0.1) | (0.07) | (0.14) | (0.09) | (0.06) | (0.17) | (0.09) | ||
| FL | 2.3 | 2.5* | 3.8* | 18.9* | 27.8* | 11.7 | 10.0 | 7.6 | 14.2 | 20.4 | 21.3 | 21.1 | 22.8 | |
| (0.28) | (0.20) | (0.56) | (3.38) | (3.33) | (3.17) | (2.26) | (1.64) | (4.26) | (7.60) | (8.27) | (17.0) | (17.0) | ||
| Lymphocyte (×10−3/μL) | Control | 1.5 | 1.9 | 3.4 | 6.3 | 6.3 | 9.3 | 7.2 | 10.1 | 12.3 | 11.6 | 9.8 | 7.3 | 12.3 |
| (0.14) | (0.14) | (0.16) | (0.32) | (0.52) | (1.0) | (0.61) | (1.05) | (1.06) | (1.02) | (1.13) | (1.54) | (0.87) | ||
| FL | 0.9 | 4.9* | 5.4* | 10.8† | 21.5* | 18.5 | 12.9 | 14.5 | 26.0 | 29.1 | 29.0† | 25.9 | 23.7 | |
| (0.08) | (0.45) | (0.41) | (1.15) | (2.08) | (4.26) | (2.74) | (3.06) | (3.26) | (4.77) | (2.47) | (7.1) | (7.28) | ||
| Monocyte (×10−3/μL) | Control | 0.5 | 0.2 | 0.2 | 0.3 | 0.2 | 0.3 | 0.2 | 0.3 | 0.4 | 0.3 | 0.3 | 0.3 | 0.5 |
| (0.07) | (0.03) | (0.01) | (0.02) | (0.01) | (0.03) | (0.02) | (0.02) | (0.04) | (0.04) | (0.04) | (0.11) | (0.11) | ||
| FL | 0.2 | 0.7* | 0.8* | 2.4* | 4.2* | 7.7 | 1.7† | 2.6† | 4.7† | 6.9 | 5.6 | 4.2 | 3.1 | |
| (0.02) | (0.08) | (0.06) | (0.22) | (0.42) | (5.45) | (0.33) | (0.46) | (0.9) | (1.93) | (1.23) | (2.35) | (1.66) | ||
| Eosinophil (×10−3/μL) | Control | 0.02 | 0.38 | 0.12 | 0.16 | 0.17 | 0.31 | 0.25 | 0.23 | 0.27 | 0.22 | 0.27 | 0.15 | 0.36 |
| (0.002) | (0.1) | (0.01) | (0.02) | (0.03) | (0.08) | (0.06) | (0.03) | (0.03) | (0.04) | (0.04) | (0.04) | (0.02) | ||
| FL | 0.01 | 0.13 | 0.40 | 0.33 | 0.22 | 0.10 | 0.12 | 0.42 | 0.45 | 0.42 | 0.40 | 0.18 | 0.12 | |
| (0.002) | (0.03) | (0.04) | (0.04) | (0.04) | (0.04) | (0.03) | (0.09) | (0.10) | (0.04) | (0.02) | (0.04) | (0.03) | ||
| Basophil (×10−3/μL) | Control | 0.02 | 0.01 | 0.03 | 0.04 | 0.03 | 0.04 | 0.04 | 0.05 | 0.11 | 0.06 | 0.09 | 0.03 | 0.05 |
| (0.004) | (0.002) | (0.003) | (0.01) | (0.003) | (0.01) | (0.01) | (0.01) | (0.03) | (0.02) | (0.02) | (0.01) | (0.01) | ||
| FL | 0.01 | 0.06* | 0.08* | 0.28* | 0.54* | 0.28† | 0.17 | 0.30 | 0.79 | 0.70 | 0.61 | 0.53 | 0.44 | |
| (0.002) | (0.01) | (0.01) | (0.04) | (0.09) | (0.06) | (0.05) | (0.1) | (0.31) | (0.29) | (0.17) | (0.38) | (0.32) | ||
| LUCs (×10−3/μL) | Control | 0.1 | 0.03 | 0.03 | 0.09 | 0.08 | 0.04 | 0.07 | 0.13 | 0.15 | 0.15 | 0.13 | 0.04 | 0.23 |
| (0.01) | (0.004) | (0.004) | (0.02) | (0.01) | (0.02) | (0.01) | (0.02) | (0.03) | (0.02) | (0.03) | (0.01) | (0.12) | ||
| FL | 0.06 | 0.27† | 0.27* | 0.77* | 2.06* | 0.15 | 0.86 | 1.07 | 1.14 | 2.38 | 1.80 | 1.01 | 2.05 | |
| (0.01) | (0.06) | (0.04) | (0.12) | (0.24) | (0.03) | (0.28) | (0.35) | (0.29) | (0.97) | (0.63) | (0.73) | (1.67) | ||
Peripheral blood mononuclear cells from FL-overexpressing and control animals were differentially counted as a function of time posttransplantation, as described. Neutrophils, lymphocytes, monocytes, basophils, eosinophils, and LUCs were enumerated. LUCs are considered morphologically to be lymphoblast-like cells. Results are from a representative experiment. The number of animals analyzed (n) in this experiment is as follows: n = 10 for each group from 0 to 5 weeks posttransplantation, n = 5 in the control group for weeks 6 through 13 posttransplantation, n =5 in the FL-overexpressing group from week 6 through 9 posttransplantation, n = 3 in the FL-overexpressing group from week 10 through 11 posttransplantation, and n = 2 in the FL-overexpressing group from week 12 through 13 posttransplantation. The data are expressed as an arithmetic mean of cell counts (±1 SEM). The significance of difference between FL-expressing and control animals at each time point was determined using the Student's t-test. H1E differential counts were validated by direct examination of blood smears (data not shown).
P < .001.
.005 < P < .001.