Effect of PAF Treatment on the Ability of BMSC to Support CFU-GM Colony Formation From Autologous CD34+ Cells
| Culture Conditions . | Exp 1 . | Exp 2 . | Exp 3 . | Exp 4 . |
|---|---|---|---|---|
| Control | 15 ± 1* | 39 ± 6 | 19 ± 1 | 17 ± 7 |
| PAF | 39 ± 5 | 52 ± 9 | 14 ± 6 | 22 ± 2 |
| WEB2170 | 16 ± 3 | 41 ± 4 | 11 ± 4 | 21 ± 4 |
| PAF + WEB2170 | 16 ± 2 | 45 ± 2 | 10 ± 4 | 21 ± 2 |
| Control + anti-PRL | 17 ± 3 | 33 ± 4 | 10 ± 3 | 18 ± 5 |
| PAF + anti-PRL | 24 ± 4 | 49 ± 7 | 19 ± 2 | 24 ± 4 |
| WEB2170 + anti-PRL | 18 ± 2 | 38 ± 6 | 11 ± 2 | 19 ± 3 |
| PAF + WEB2170 + anti-PRL | 26 ± 3 | 40 ± 4 | 11 ± 7 | 20 ± 2 |
| Culture Conditions . | Exp 1 . | Exp 2 . | Exp 3 . | Exp 4 . |
|---|---|---|---|---|
| Control | 15 ± 1* | 39 ± 6 | 19 ± 1 | 17 ± 7 |
| PAF | 39 ± 5 | 52 ± 9 | 14 ± 6 | 22 ± 2 |
| WEB2170 | 16 ± 3 | 41 ± 4 | 11 ± 4 | 21 ± 4 |
| PAF + WEB2170 | 16 ± 2 | 45 ± 2 | 10 ± 4 | 21 ± 2 |
| Control + anti-PRL | 17 ± 3 | 33 ± 4 | 10 ± 3 | 18 ± 5 |
| PAF + anti-PRL | 24 ± 4 | 49 ± 7 | 19 ± 2 | 24 ± 4 |
| WEB2170 + anti-PRL | 18 ± 2 | 38 ± 6 | 11 ± 2 | 19 ± 3 |
| PAF + WEB2170 + anti-PRL | 26 ± 3 | 40 ± 4 | 11 ± 7 | 20 ± 2 |
Abbreviations: anti-PRL, rabbit antiserum to human prolactin; WEB2170, PAF receptor antagonist.
Data represent the mean ± SD number of CFU-GM colonies per well in triplicate cultures each seeded with 5 × 102 cells. Cultures were performed in the presence of IL-3, GM-CSF, and Epo. No nonspecific inhibition was observed when using a nonimmune rabbit antiserum (not shown).