Effect of PAF Treatment on the Ability of BMSC to Support BFU-E Colony Formation From Autologous CD34+ Cells
| Culture Conditions . | Exp 1 . | Exp 2 . | Exp 3 . | Exp 4 . |
|---|---|---|---|---|
| Control | 29 ± 2* | 45 ± 8 | 34 ± 8 | 24 ± 3 |
| PAF | 55 ± 5 | 72 ± 7 | 50 ± 7 | 39 ± 4 |
| WEB2170 | 31 ± 3 | 49 ± 5 | 37 ± 7 | 24 ± 1 |
| PAF + WEB2170 | 28 ± 3 | 43 ± 6 | 48 ± 3 | 30 ± 3 |
| Control + anti-PRL | 25 ± 4 | 39 ± 5 | 31 ± 7 | 20 ± 1 |
| PAF + anti-PRL | 33 ± 3 | 56 ± 4 | 46 ± 3 | 27 ± 6 |
| WEB2170 + anti-PRL | 26 ± 2 | 40 ± 5 | 36 ± 11 | 24 ± 5 |
| PAF + WEB2170 + anti-PRL | 27 ± 5 | 46 ± 7 | 35 ± 10 | 23 ± 4 |
| Culture Conditions . | Exp 1 . | Exp 2 . | Exp 3 . | Exp 4 . |
|---|---|---|---|---|
| Control | 29 ± 2* | 45 ± 8 | 34 ± 8 | 24 ± 3 |
| PAF | 55 ± 5 | 72 ± 7 | 50 ± 7 | 39 ± 4 |
| WEB2170 | 31 ± 3 | 49 ± 5 | 37 ± 7 | 24 ± 1 |
| PAF + WEB2170 | 28 ± 3 | 43 ± 6 | 48 ± 3 | 30 ± 3 |
| Control + anti-PRL | 25 ± 4 | 39 ± 5 | 31 ± 7 | 20 ± 1 |
| PAF + anti-PRL | 33 ± 3 | 56 ± 4 | 46 ± 3 | 27 ± 6 |
| WEB2170 + anti-PRL | 26 ± 2 | 40 ± 5 | 36 ± 11 | 24 ± 5 |
| PAF + WEB2170 + anti-PRL | 27 ± 5 | 46 ± 7 | 35 ± 10 | 23 ± 4 |
Abbreviations: anti-PRL, rabbit antiserum to human prolactin; WEB2170, PAF receptor antagonist.
Data represent the mean ± SD number of BFU-E colonies per well in triplicate cultures each seeded with 5 × 102 cells. Cultures were performed in the presence of IL-3, GM-CSF, and Epo. No nonspecific inhibition was observed when using a nonimmune rabbit antiserum (not shown).