Expression of GM-CSFR on Human Cells
| Cell Line . | Sites/Cell . | kd (nmol/L) . |
|---|---|---|
| LS174T | 900 ± 700 | 1.7 ± 1 |
| SW403 | 3,050 ± 900 | 1.6 ± 0.5 |
| N87 | 2,400 ± 1,200 | 0.9 ± 0.7 |
| HTB-103 | 500 ± 200 | 0.4 ± 0.4 |
| HL60 | 540 ± 50 | 0.2 ± 0.01 |
| TF-1 | 3,700 ± 1,500 | 0.5 ± 0.05 |
| U937 | 3,500 ± 350 | 1.9 ± 0.2 |
| Cell Line . | Sites/Cell . | kd (nmol/L) . |
|---|---|---|
| LS174T | 900 ± 700 | 1.7 ± 1 |
| SW403 | 3,050 ± 900 | 1.6 ± 0.5 |
| N87 | 2,400 ± 1,200 | 0.9 ± 0.7 |
| HTB-103 | 500 ± 200 | 0.4 ± 0.4 |
| HL60 | 540 ± 50 | 0.2 ± 0.01 |
| TF-1 | 3,700 ± 1,500 | 0.5 ± 0.05 |
| U937 | 3,500 ± 350 | 1.9 ± 0.2 |
Cells were incubated at 37°C for 30 minutes with [125I]–GM-CSF with or without a 100-fold excess of GM-CSF in RPMI media containing 1 mg/mL bovine serum albumin and 0.2% NaN3. Unbound GM-CSF was removed from the cells either by centrifuging through n-butyl phthalate or by centrifuging and washing the cells with binding buffer.