CMV-Induced Factor(s) Impairs the Lymphocyte Proliferative Response to Mitogens and Recall Antigen
| . | Mock Preparation . | Cell-Free HCMV . | Inhibition (%) . | P Value . | Mock CM . | CMV CM . | Inhibition (%) . | P Value . |
|---|---|---|---|---|---|---|---|---|
| Mitogens (day 3) | ||||||||
| Control medium | 2.2 ± 1.23-150 | 1.7 ± 0.83-150 | 2.4 ± 0.63-150 | 2.0 ± 1.03-150 | ||||
| PHA | 193.9 ± 16.1 | 137.2 ± 11.7 | 29 ± 13-151 | <.0013-152 | 189.2 ± 19.8 | 130.5 ± 22.8 | 31 ± 53-151 | <.0013-152 |
| PWM | 58.7 ± 15.5 | 45.1 ± 11.2 | 23 ± 5 | .026 | 63.0 ± 16.5 | 43.3 ± 12.5 | 32 ± 4 | .005 |
| Recall antigen (day 6) | ||||||||
| Control medium | 33.9 ± 18.0 | 15.2 ± 9.2 | 45.4 ± 19.4 | 11.2 ± 6.5 | ||||
| TT | 124.4 ± 15.1 | 61.5 ± 16.9 | 51 ± 9 | <.001 | 121.9 ± 18.6 | 80.1 ± 16.5 | 35 ± 6 | <.001 |
| . | Mock Preparation . | Cell-Free HCMV . | Inhibition (%) . | P Value . | Mock CM . | CMV CM . | Inhibition (%) . | P Value . |
|---|---|---|---|---|---|---|---|---|
| Mitogens (day 3) | ||||||||
| Control medium | 2.2 ± 1.23-150 | 1.7 ± 0.83-150 | 2.4 ± 0.63-150 | 2.0 ± 1.03-150 | ||||
| PHA | 193.9 ± 16.1 | 137.2 ± 11.7 | 29 ± 13-151 | <.0013-152 | 189.2 ± 19.8 | 130.5 ± 22.8 | 31 ± 53-151 | <.0013-152 |
| PWM | 58.7 ± 15.5 | 45.1 ± 11.2 | 23 ± 5 | .026 | 63.0 ± 16.5 | 43.3 ± 12.5 | 32 ± 4 | .005 |
| Recall antigen (day 6) | ||||||||
| Control medium | 33.9 ± 18.0 | 15.2 ± 9.2 | 45.4 ± 19.4 | 11.2 ± 6.5 | ||||
| TT | 124.4 ± 15.1 | 61.5 ± 16.9 | 51 ± 9 | <.001 | 121.9 ± 18.6 | 80.1 ± 16.5 | 35 ± 6 | <.001 |
The proliferative response of PBMC incubated with cell-free inactivated HCMV was compared with the proliferative response of PBMC incubated with CMV CM. The inactivated HCMV and the mock preparation or the day 2 CMV CM and the mock CM were added at day 0 and maintained in culture.
3H-thymidine incorporation assays are shown as the mean cpm/well ± SD multiplied per 103, obtained with 3 different PBMC cultures from HCMV seronegative donors. Each assay was done in triplicate.
Percentage of inhibition = 100 minus (value obtained with HCMV or CMV CM/its mock control multiplied by 100).
P value, comparing the proliferative response obtained with cell-free HCMV or CMV CM with their respective mock control.