Effects of PF4 on Apoptosis Induced by TGFβ1, 5-FU, or ETP in CD34+ CB Cells
| PF4 ( μg/mL) . | TGFβ1 (1 ng/mL) . | 5-FU (2.5 μg/mL) . | Etoposide (1 μg/mL) . | |||
|---|---|---|---|---|---|---|
| . | Viability (%) . | Apoptotic Cells (%) . | Viability (%) . | Apoptotic Cells (%) . | Viability (%) . | Apoptotic Cells (%) . |
| 0 | 72 ± 6 | 10 ± 3 | 45 ± 3 | 17 ± 2 | 41 ± 3 | 21 ± 3 |
| 5 | 91 ± 34-150 | 2 ± 14-150 | 87 ± 64-150 | 3 ± 24-150 | 79 ± 64-150 | 4 ± 14-150 |
| PF4 ( μg/mL) . | TGFβ1 (1 ng/mL) . | 5-FU (2.5 μg/mL) . | Etoposide (1 μg/mL) . | |||
|---|---|---|---|---|---|---|
| . | Viability (%) . | Apoptotic Cells (%) . | Viability (%) . | Apoptotic Cells (%) . | Viability (%) . | Apoptotic Cells (%) . |
| 0 | 72 ± 6 | 10 ± 3 | 45 ± 3 | 17 ± 2 | 41 ± 3 | 21 ± 3 |
| 5 | 91 ± 34-150 | 2 ± 14-150 | 87 ± 64-150 | 3 ± 24-150 | 79 ± 64-150 | 4 ± 14-150 |
CD34+ CB cells at 104/mL were first incubated in liquid culture in the presence or absence of PF4 5 μg/mL for 2 days. TGFβ1, 5-FU, or ETP were separately added to the culture for an additional incubation for 24 hours. Results are expressed as the mean ± SEM of triplicate determinations.
P < .05 v control cells without PF4 treatment.