Chemoprotective Effect on Normal Bone Marrow Cells and Two Tumor Cell Lines, SMMC 7721 and MKN-45
| Drug . | Cell Viability (%) . | ||
|---|---|---|---|
| . | Bone Marrow . | SMMC 7721 . | MKN-45 . |
| None | 100 ± 9.2 | 100 ± 15 | 100 ± 8 |
| PF4 | 143 ± 133-151 | 101 ± 15 | 96 ± 1 |
| DNR | 34 ± 5 | 48 ± 4 | 54 ± 12 |
| DNR + PF4 | 64 ± 83-150 | 47 ± 4 | 49 ± 10 |
| ETP | 27 ± 3 | 40 ± 7 | 51 ± 9 |
| ETP + PF4 | 63 ± 53-150 | 43 ± 4 | 56 ± 5 |
| Drug . | Cell Viability (%) . | ||
|---|---|---|---|
| . | Bone Marrow . | SMMC 7721 . | MKN-45 . |
| None | 100 ± 9.2 | 100 ± 15 | 100 ± 8 |
| PF4 | 143 ± 133-151 | 101 ± 15 | 96 ± 1 |
| DNR | 34 ± 5 | 48 ± 4 | 54 ± 12 |
| DNR + PF4 | 64 ± 83-150 | 47 ± 4 | 49 ± 10 |
| ETP | 27 ± 3 | 40 ± 7 | 51 ± 9 |
| ETP + PF4 | 63 ± 53-150 | 43 ± 4 | 56 ± 5 |
Cells (2 × 106/mL) were preincubated with or without PF4 (5 μg/mL) in a volume of 100 μL per well in 24-well culture plates for 48 hours. DNR 1 μg/mL and ETP 1 μg/mL were then added respectively for an additional 24 hours. Cell viability was measured with an MTT assay. Data are expressed as the mean ± SEM from triplicate determinations of 3 separate experiments. 100% corresponds to the number of viable cells in drug- and PF4-free control samples after incubation for 3 days.
P < .05 v drug.
P < .01 v control group.