BIAcore Analysis of MoAb34 and Fab34 Interactions With EPObp
| Immobilized Molecule . | Analyte . | KA (mol/L−1⋅s−1) . | kd (s−1) . | KD (nmol/L) . |
|---|---|---|---|---|
| MoAb34 | EPObp | 3.1 × 105 | 0.026 | 84 |
| EPObp | Fab34 | 4.9 × 105 | 0.026 | 53 |
| Immobilized Molecule . | Analyte . | KA (mol/L−1⋅s−1) . | kd (s−1) . | KD (nmol/L) . |
|---|---|---|---|---|
| MoAb34 | EPObp | 3.1 × 105 | 0.026 | 84 |
| EPObp | Fab34 | 4.9 × 105 | 0.026 | 53 |
Determinations of affinity were made from kinetic measurements of KA and kd , and KD was inferred from the relationship KD = kd/KA . Standard errors in all cases were <10%.