Induction of primary CTLs from healthy donors after stimulation with HM1.24 peptide–pulsed DCs
. | HLA-A2+, A24+ . | . | . | . | HLA-A2+, A24- . | . | . | HLA-A2-, A24+ . | . | . | . | . | . | . | . | . | . | . | HLA-A2-, A24- . | . | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Peptide . | 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 9 . | 10 . | 11 . | 12 . | 13 . | 14 . | 15 . | 16 . | 17 . | 18 . | 19 . | 20 . | ||||||||||||||||
| HM1.24-126 | 0 | 35 | 0 | 29 | 0 | 25 | 38 | 0 | 0 | 2 | 3 | 0 | 0 | 0 | 30 | 0 | 26 | 0 | 0 | 0 | ||||||||||||||||
| HM1.24-136 | 1 | 6 | 3 | 0 | 0 | 0 | 7 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 33 | 1 | 0 | 0 | ||||||||||||||||
| HM1.24-152 | 0 | 0 | 0 | 0 | 0 | 0 | 24 | 0 | 5 | 0 | 0 | 0 | 3 | 44 | 0 | 8 | 2 | 0 | 0 | 0 | ||||||||||||||||
| HM1.24-165 | 31 | 25 | 3 | 0 | 26 | 0 | 20 | 29 | 35 | 1 | 0 | 1 | 13 | 7 | 36 | 8 | 16 | 1 | 0 | 0 | ||||||||||||||||
| MAGE-3-195 | 2 | 35 | 0 | 37 | 0 | 0 | 1 | 38 | 2 | 0 | 0 | 0 | 28 | 0 | 20 | 19 | 3 | 3 | 0 | 0 | ||||||||||||||||
. | HLA-A2+, A24+ . | . | . | . | HLA-A2+, A24- . | . | . | HLA-A2-, A24+ . | . | . | . | . | . | . | . | . | . | . | HLA-A2-, A24- . | . | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Peptide . | 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 9 . | 10 . | 11 . | 12 . | 13 . | 14 . | 15 . | 16 . | 17 . | 18 . | 19 . | 20 . | ||||||||||||||||
| HM1.24-126 | 0 | 35 | 0 | 29 | 0 | 25 | 38 | 0 | 0 | 2 | 3 | 0 | 0 | 0 | 30 | 0 | 26 | 0 | 0 | 0 | ||||||||||||||||
| HM1.24-136 | 1 | 6 | 3 | 0 | 0 | 0 | 7 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 33 | 1 | 0 | 0 | ||||||||||||||||
| HM1.24-152 | 0 | 0 | 0 | 0 | 0 | 0 | 24 | 0 | 5 | 0 | 0 | 0 | 3 | 44 | 0 | 8 | 2 | 0 | 0 | 0 | ||||||||||||||||
| HM1.24-165 | 31 | 25 | 3 | 0 | 26 | 0 | 20 | 29 | 35 | 1 | 0 | 1 | 13 | 7 | 36 | 8 | 16 | 1 | 0 | 0 | ||||||||||||||||
| MAGE-3-195 | 2 | 35 | 0 | 37 | 0 | 0 | 1 | 38 | 2 | 0 | 0 | 0 | 28 | 0 | 20 | 19 | 3 | 3 | 0 | 0 | ||||||||||||||||
CD8+ T cells were purified from 20 healthy donors and stimulated with autologous DCs pulsed with HM1.24 peptides or MAGE-3-195 peptide once a week. Five days after the third stimulation (day 19), cells were harvested and tested for CTL activity against YN cells that had been loaded with the relevant peptide. Cytotoxicity (%) was determined by a 4-hour 51Cr-release assay at an E/T ratio of 20. Values are shown as the mean cytotoxic activity of triplicate experiments of each individual in 4 HLA groups.