Allogeneic T-cell proliferation in the presence of MVA-TRICOM–infected CLL cells
Patient, stimulator cells . | Allogeneic proliferation stimulation index . |
|---|---|
| 1 | |
| Uninfected | 1.0 |
| MVA-WT | 1.3 |
| MVA-TRICOM | 131.0 |
| 2 | |
| Uninfected | 0.6 |
| MVA-WT | 0.7 |
| MVA-TRICOM | 121.8 |
| 4 | |
| Uninfected | 0.6 |
| MVA-WT | 0.8 |
| MVA-TRICOM | 68.6 |
| 16 | |
| Uninfected | 11.7 |
| MVA-WT | 9.4 |
| MVA-TRICOM | 34.4 |
| 17 | |
| Uninfected | 2.9 |
| MVA-WT | 2.9 |
| MVA-TRICOM | 179.6 |
| 18 | |
| Uninfected | 9.0 |
| MVA-WT | 3.1 |
| MVA-TRICOM | 35.8 |
Patient, stimulator cells . | Allogeneic proliferation stimulation index . |
|---|---|
| 1 | |
| Uninfected | 1.0 |
| MVA-WT | 1.3 |
| MVA-TRICOM | 131.0 |
| 2 | |
| Uninfected | 0.6 |
| MVA-WT | 0.7 |
| MVA-TRICOM | 121.8 |
| 4 | |
| Uninfected | 0.6 |
| MVA-WT | 0.8 |
| MVA-TRICOM | 68.6 |
| 16 | |
| Uninfected | 11.7 |
| MVA-WT | 9.4 |
| MVA-TRICOM | 34.4 |
| 17 | |
| Uninfected | 2.9 |
| MVA-WT | 2.9 |
| MVA-TRICOM | 179.6 |
| 18 | |
| Uninfected | 9.0 |
| MVA-WT | 3.1 |
| MVA-TRICOM | 35.8 |
CD3+ T cells from healthy donors were stimulated with uninfected, MVA-WT– or MVA-TRICOM–infected CLL cells, at a ratio of stimulator to effector cells equal to 1:10. Proliferation was measured as 3H-thymidine incorporation on day 5 of culture. Stimulation index was calculated as cpm (T cells + CLL cells)/cpm (T cells alone).