Flow cytometry analysis of PBMCs from patients 10 and 11 at 24 hours after infection with 10 MOI V-WT, PUVA-V-WT, rV-TRICOM, or PUVA-rV-TRICOM
Patient, vector . | B7-1, % (MFI) . | ICAM-1, % (MFI) . | LFA-3, % (MFI) . |
|---|---|---|---|
| Patient 10 | |||
| Uninfected | 12.0 (22) | 19.9 (31) | 38.9 (30) |
| V-WT | 17.1 (33) | 23.7 (28) | 37.6 (22) |
| PUVA-V-WT | 23.5 (17) | 17.4 (28) | 41.4 (29) |
| rV-TRICOM | 39.9 (130) | 54.9 (483) | 49.6 (112) |
| PUVA-rV-TRICOM | 27.5 (116) | 27.5 (125) | 39.5 (39) |
| Patient 11 | |||
| Uninfected | 18.1 (31) | 81.1 (62) | 48.4 (32) |
| V-WT | 15.4 (34) | 76.7 (37) | 36.1 (20) |
| PUVA-V-WT | 13.6 (43) | 75.7 (29) | 45.0 (19) |
| rV-TRICOM | 52.8 (237) | 96.3 (1055) | 75.1 (168) |
| PUVA-rV-TRICOM | 38.3 (129) | 89.1 (414) | 51.9 (73) |
Patient, vector . | B7-1, % (MFI) . | ICAM-1, % (MFI) . | LFA-3, % (MFI) . |
|---|---|---|---|
| Patient 10 | |||
| Uninfected | 12.0 (22) | 19.9 (31) | 38.9 (30) |
| V-WT | 17.1 (33) | 23.7 (28) | 37.6 (22) |
| PUVA-V-WT | 23.5 (17) | 17.4 (28) | 41.4 (29) |
| rV-TRICOM | 39.9 (130) | 54.9 (483) | 49.6 (112) |
| PUVA-rV-TRICOM | 27.5 (116) | 27.5 (125) | 39.5 (39) |
| Patient 11 | |||
| Uninfected | 18.1 (31) | 81.1 (62) | 48.4 (32) |
| V-WT | 15.4 (34) | 76.7 (37) | 36.1 (20) |
| PUVA-V-WT | 13.6 (43) | 75.7 (29) | 45.0 (19) |
| rV-TRICOM | 52.8 (237) | 96.3 (1055) | 75.1 (168) |
| PUVA-rV-TRICOM | 38.3 (129) | 89.1 (414) | 51.9 (73) |
Percentages are for cells that were positive for CD19 and each costimulatory molecule (B7-1, ICAM-1, and LFA-3). MFI values are for the expression of each costimulatory molecule.