Table 1. CFU-F and CFU-O colony... | American Society of Hematology

Table 1.

CFU-F and CFU-O colony numbers/10⁴ cells from suspension-derived cells of various cell fractions

	CFU-F							CFU-O
Time, d	Unsorted cells	CD123⁺CD45^- cells*	CD45^- cells sorted†	CD123⁺CD45^- cells‡	Unsorted cells	CD123⁺CD45^- cells*	CD45^- cells sorted†	CD123⁺CD45^- cells‡
0	10 ± 2	0 ± 0	8 ± 0	0 ± 0	5 ± 2	0 ± 0	4 ± 0	0 ± 0
7	14 ± 2	2.3 ± 0.6	1 ± 0	0.3 ± 0.6	8.6 ± 1.5	0.67 ± 0	0.7 ± 0.6	0.3 ± 0.6
14	11 ± 4	3 ± 1	1.3 ± 0.6	1.0 ± 0	15 ± 3	1.33 ± 0.6	2.3 ± 0.6	0.3 ± 0.6
21	19 ± 5	4.3 ± 0.6	7 ± 1	1.7 ± 0.6	20 ± 3.5	3.6 ± 1	3 ± 0.6	0.67 ± 0.6

	CFU-F							CFU-O
Time, d	Unsorted cells	CD123⁺CD45^- cells*	CD45^- cells sorted†	CD123⁺CD45^- cells‡	Unsorted cells	CD123⁺CD45^- cells*	CD45^- cells sorted†	CD123⁺CD45^- cells‡
0	10 ± 2	0 ± 0	8 ± 0	0 ± 0	5 ± 2	0 ± 0	4 ± 0	0 ± 0
7	14 ± 2	2.3 ± 0.6	1 ± 0	0.3 ± 0.6	8.6 ± 1.5	0.67 ± 0	0.7 ± 0.6	0.3 ± 0.6
14	11 ± 4	3 ± 1	1.3 ± 0.6	1.0 ± 0	15 ± 3	1.33 ± 0.6	2.3 ± 0.6	0.3 ± 0.6
21	19 ± 5	4.3 ± 0.6	7 ± 1	1.7 ± 0.6	20 ± 3.5	3.6 ± 1	3 ± 0.6	0.67 ± 0.6

CD123⁺CD45^- cells isolated from unsorted cells that were grown in stirred suspension.

CD45^- cells were sorted from input BM samples and were directly grown in stirred suspension.

CD123⁺CD45^- cells isolated from CD45^--sorted cells that were grown in stirred suspension.