Nested mtDNA PCR efficiency of single CD34+ cells, lymphocytes and granulocytes from healthy donors
. | . | PCR efficiency . | . | . | . | . | . | . | . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | CD34+ cells . | . | T cells . | . | B cells . | . | Granulocytes . | . | |||||||
| Donor no. . | Age, y/sex . | No. total . | No. positive (%) . | No. total . | No. positive (%) . | No. total . | No. positive (%) . | No. total . | No. positive (%) . | |||||||
| 1 | 48/F | 96 | 85 (88.5) | 96 | 88 (91.7) | 96 | 87 (90.6) | 96 | 66 (68.8) | |||||||
| 2 | 44/M | 96 | 92 (95.8) | 96 | 94 (97.9) | 96 | 78 (81.2) | 96 | 82 (85.4) | |||||||
| 3 | 36/M | 96 | 94 (97.9) | 96 | 86 (89.6) | 96 | 94 (97.9) | 96 | 72 (75.0) | |||||||
| 4 | 55/M | 96 | 93 (96.9) | 96 | 94 (97.9) | 96 | 93 (96.9) | 96 | 77 (80.2) | |||||||
| 5 | 35/F | 96 | 93 (96.9) | 96 | 96 (100.0) | 96 | 93 (96.9) | 96 | 84 (87.5) | |||||||
| Total | NA/NA | 480 | 457 (95.2) | 480 | 458 (95.4) | 480 | 445 (92.7) | 480 | 381 (79.4) | |||||||
. | . | PCR efficiency . | . | . | . | . | . | . | . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | CD34+ cells . | . | T cells . | . | B cells . | . | Granulocytes . | . | |||||||
| Donor no. . | Age, y/sex . | No. total . | No. positive (%) . | No. total . | No. positive (%) . | No. total . | No. positive (%) . | No. total . | No. positive (%) . | |||||||
| 1 | 48/F | 96 | 85 (88.5) | 96 | 88 (91.7) | 96 | 87 (90.6) | 96 | 66 (68.8) | |||||||
| 2 | 44/M | 96 | 92 (95.8) | 96 | 94 (97.9) | 96 | 78 (81.2) | 96 | 82 (85.4) | |||||||
| 3 | 36/M | 96 | 94 (97.9) | 96 | 86 (89.6) | 96 | 94 (97.9) | 96 | 72 (75.0) | |||||||
| 4 | 55/M | 96 | 93 (96.9) | 96 | 94 (97.9) | 96 | 93 (96.9) | 96 | 77 (80.2) | |||||||
| 5 | 35/F | 96 | 93 (96.9) | 96 | 96 (100.0) | 96 | 93 (96.9) | 96 | 84 (87.5) | |||||||
| Total | NA/NA | 480 | 457 (95.2) | 480 | 458 (95.4) | 480 | 445 (92.7) | 480 | 381 (79.4) | |||||||
NA indicates not applicable.