Phenotypes of mice fed normal or iron-deficient diet for 8 weeks
. | Male mice . | . | Female mice . | . | . | |||
|---|---|---|---|---|---|---|---|---|
. | WT, n = 3 . | Cybrdl−/−, n = 3 . | WT, n = 7 . | WT, n = 10 . | Cybrdl−/−, n = 9 . | |||
| Diet | Normal | Normal | Normal | Fe (−) | Fe (−) | |||
| Body weight, g | 30.3 ± 1.1 | 26.5 ± 1.0 | 23.0 ± 0.81* | 19.9 ± 0.62 | 20.2 ± 1.12 | |||
| Liver Fe, μg/g | 82.3 ± 7.6 | 79.2 ± 4.1 | 188.5 ± 26.0* | 86.3 ± 9.1 | 67.8 ± 7.3 | |||
| Spleen Fe, μg/g | 1031 ± 316 | 1071 ± 283 | 1248.3 ± 190.1* | 731.3 ± 101.1 | 655.7 ± 67.2 | |||
| Hemoglobin, g/L | 145 ± 3 | 156 ± 2† | 142 ± 2 | 142 ± 2 | 142 ± 3 | |||
. | Male mice . | . | Female mice . | . | . | |||
|---|---|---|---|---|---|---|---|---|
. | WT, n = 3 . | Cybrdl−/−, n = 3 . | WT, n = 7 . | WT, n = 10 . | Cybrdl−/−, n = 9 . | |||
| Diet | Normal | Normal | Normal | Fe (−) | Fe (−) | |||
| Body weight, g | 30.3 ± 1.1 | 26.5 ± 1.0 | 23.0 ± 0.81* | 19.9 ± 0.62 | 20.2 ± 1.12 | |||
| Liver Fe, μg/g | 82.3 ± 7.6 | 79.2 ± 4.1 | 188.5 ± 26.0* | 86.3 ± 9.1 | 67.8 ± 7.3 | |||
| Spleen Fe, μg/g | 1031 ± 316 | 1071 ± 283 | 1248.3 ± 190.1* | 731.3 ± 101.1 | 655.7 ± 67.2 | |||
| Hemoglobin, g/L | 145 ± 3 | 156 ± 2† | 142 ± 2 | 142 ± 2 | 142 ± 3 | |||
Because iron storage parameters are known to differ between male and female mice, each experiment was performed using sex-matched animals. Measurements (mean ± SEM) are given for 12-week-old male and female wild-type (WT) and Cybrdl−/− mice. Statistical analysis was performed using analysis of variance between groups (ANOVA). Tissue nonheme iron content is indicated as μg/g wet weight of the specimen. Body weight and tissue iron levels were significantly greater in female WT mice on normal diet than in female of either genotype on an iron-deficient diet. While there was a trend toward lower tissue iron content in Cybrdl−/− mice on an iron-deficient diet compared with WT mice on an iron-deficient diet in both females and males, the differences were not significant.
Fe (−) diet indicates an iron-deficient diet.
P < .05.
Male WT mice had significantly lower hemoglobin levels compared with Cybrdl−/− mice.