Distribution of MTHFR genotypes in childhood ALL patients and controls
Genotype . | ALL cases, no. (%) . | Controls, no. (%) . | OR*(95% CI) . | P . |
|---|---|---|---|---|
| MTHFR C677T genotype† | ||||
| CC | 199 (43.9) | 600 (41.4) | 1.00 (referent) | - |
| CT | 195 (43.0) | 681 (47.0) | 0.86 (0.7-1.1) | .2 |
| TT | 59 (13.0) | 167 (11.5) | 1.07 (0.8-1.6) | .8 |
| CT + TT | 254 (56.1) | 848 (58.6) | 0.90 (0.7-1.1) | .4 |
| C-allele | 593 (65.5) | 1881 (65.0) | 1.00 (referent) | - |
| T-allele | 313 (34.5) | 1015 (35.0) | 0.98 (0.8-1.2) | .8 |
| MTHFR A1298C genotype‡ | ||||
| AA | 198 (44.5) | 660 (45.4) | 1.00 (referent) | - |
| AC | 195 (43.0) | 644 (44.3) | 1.01 (0.8-1.3) | 1 |
| CC | 52 (11.7) | 149 (10.2) | 1.16 (0.8-1.7) | .5 |
| AC + CC | 247 (55.5) | 793 (54.5) | 1.04 (0.8-1.3) | .8 |
| A-allele | 591 (66.4) | 1964 (67.6) | 1.00 (referent) | - |
| C-allele | 299 (33.6) | 942 (32.4) | 1.05 (0.9-1.2) | .5 |
| Genotype combination | ||||
| C677T/A1298C§ | ||||
| CC/AA | 45 (10.2) | 150 (10.5) | 1.00 (referent)∥ | - |
| CC/AC | 98 (22.3) | 296 (20.6) | 1.10 (0.7-1.7) | .7 |
| CC/CC | 51 (11.6) | 149 (10.4) | 1.14 (0.7-1.9) | .7 |
| CT/AA | 93 (21.2) | 339 (23.6) | 0.91 (0.6-1.4)) | .7 |
| CT/AC | 95 (21.6) | 335 (23.3) | 0.95 (0.6-1.4) | .6 |
| CT/CC | 1 (0.02) | NE | - | |
| TT/AA | 57 (13.0) | 166 (11.6) | 1.14 (0.7-1.8) | .5 |
Genotype . | ALL cases, no. (%) . | Controls, no. (%) . | OR*(95% CI) . | P . |
|---|---|---|---|---|
| MTHFR C677T genotype† | ||||
| CC | 199 (43.9) | 600 (41.4) | 1.00 (referent) | - |
| CT | 195 (43.0) | 681 (47.0) | 0.86 (0.7-1.1) | .2 |
| TT | 59 (13.0) | 167 (11.5) | 1.07 (0.8-1.6) | .8 |
| CT + TT | 254 (56.1) | 848 (58.6) | 0.90 (0.7-1.1) | .4 |
| C-allele | 593 (65.5) | 1881 (65.0) | 1.00 (referent) | - |
| T-allele | 313 (34.5) | 1015 (35.0) | 0.98 (0.8-1.2) | .8 |
| MTHFR A1298C genotype‡ | ||||
| AA | 198 (44.5) | 660 (45.4) | 1.00 (referent) | - |
| AC | 195 (43.0) | 644 (44.3) | 1.01 (0.8-1.3) | 1 |
| CC | 52 (11.7) | 149 (10.2) | 1.16 (0.8-1.7) | .5 |
| AC + CC | 247 (55.5) | 793 (54.5) | 1.04 (0.8-1.3) | .8 |
| A-allele | 591 (66.4) | 1964 (67.6) | 1.00 (referent) | - |
| C-allele | 299 (33.6) | 942 (32.4) | 1.05 (0.9-1.2) | .5 |
| Genotype combination | ||||
| C677T/A1298C§ | ||||
| CC/AA | 45 (10.2) | 150 (10.5) | 1.00 (referent)∥ | - |
| CC/AC | 98 (22.3) | 296 (20.6) | 1.10 (0.7-1.7) | .7 |
| CC/CC | 51 (11.6) | 149 (10.4) | 1.14 (0.7-1.9) | .7 |
| CT/AA | 93 (21.2) | 339 (23.6) | 0.91 (0.6-1.4)) | .7 |
| CT/AC | 95 (21.6) | 335 (23.3) | 0.95 (0.6-1.4) | .6 |
| CT/CC | 1 (0.02) | NE | - | |
| TT/AA | 57 (13.0) | 166 (11.6) | 1.14 (0.7-1.8) | .5 |
- indicates none; NE, not estimated.
Allelic and genotype frequencies for both polymorphisms and frequencies of genotype combinations in cases and controls were compared by Yates corrected χ2-test. Odds ratios (ORs) for genotype frequencies between cases and controls and 95% confidence intervals (95% CI) were calculated by logistic regression using SAS version 8.2 software (SAS Institute, Heidelberg, Germany).
ALL (n = 453); controls (n = 1448). The number of cases recruited in the study was 460; however, genotype results could not be determined in 7 cases for C677T and in 15 cases for A 1298C polymorphisms. Similarly, of 1472 controls, genotype for. C677T and A 1298C polymorphisms could not be determined in 24 and 19 individuals, respectively.
ALL (n = 445); controls (n = 1453).
ALL (n = 440); controls (n = 1435).
The risk of CC677/AA 1298 individuals compared with all other genotypes is 0.98 (95% CI, 0.7-1.4; P = 96); no individual with TT677/AC 1298 and TT677/CC 1298 genotype combinations was observed.