Table 1. Effect of flavopiridol on the... | American Society of Hematology

Table 1.

Effect of flavopiridol on the expression of apoptotic genes 24 and 48 hours after incubation of CLL cells with 3 μM flavopiridol

	Effect of flavopiridol on the expression of apoptotic genes, % of control
	Mcl-1			Bcl-2			XIAP			BAG-1
Pt no. by h	mRNA	Protein	mRNA	Protein	mRNA	Protein	mRNA	pBAG-1L	pBAG-1S
24 h
1	44	2	26	36	104	4	64	74	58
2^*	62	33	54	82	216	59	101	50	8
3	66	3	25	75	56	30	81	38	25
4	53	14	58	104	93	123	70	29	21
5	45	22	41	100	58	18	68	72	59
6	53	43	68	116	91	25	66	152	124
7	39	0	24	98	38	17	56	51	0
8^*	10	22	14	138	17	63	57	17	95
9	47	24	47	100	52	69	131	102	82
Median	47	22	41	100	58	30	68	51	58
48 h
3	63	0	30	87	47	54	101	57	29
5	27	11	17	100	21	11	39	86	44
6	92	2	183	145	132	0	197	130	78
7	39	0	24	76	33	5	56	17	0
9	81	17	82	98	43	46	159	77	49
Median	63	2	30	98	43	11	101	77	44

	Effect of flavopiridol on the expression of apoptotic genes, % of control
	Mcl-1			Bcl-2			XIAP			BAG-1
Pt no. by h	mRNA	Protein	mRNA	Protein	mRNA	Protein	mRNA	pBAG-1L	pBAG-1S
24 h
1	44	2	26	36	104	4	64	74	58
2^*	62	33	54	82	216	59	101	50	8
3	66	3	25	75	56	30	81	38	25
4	53	14	58	104	93	123	70	29	21
5	45	22	41	100	58	18	68	72	59
6	53	43	68	116	91	25	66	152	124
7	39	0	24	98	38	17	56	51	0
8^*	10	22	14	138	17	63	57	17	95
9	47	24	47	100	52	69	131	102	82
Median	47	22	41	100	58	30	68	51	58
48 h
3	63	0	30	87	47	54	101	57	29
5	27	11	17	100	21	11	39	86	44
6	92	2	183	145	132	0	197	130	78
7	39	0	24	76	33	5	56	17	0
9	81	17	82	98	43	46	159	77	49
Median	63	2	30	98	43	11	101	77	44

Patients were refractory to fludarabine.