Bone marrow transduction with tamoxifen-inducible Myc stimulates myeloid colony growth in methylcellulose colony assays
Construct . | 4-OH-T . | Myeloid cytokine colonies, no. . | Lymphoid cytokine colonies, no. . | Colonies with no cytokines, no. . |
|---|---|---|---|---|
| MycER | + | 930 ± 50 | 57 ± 3 | 207 ± 18 |
| MycER | − | 390 ± 50 | 64 ± 6 | 41 ± 5 |
| GFP | + | 410 ± 40 | 34 ± 5 | 5 ± 1 |
| GFP | − | 440 ± 30 | 35 ± 3 | 8 ± 1 |
Construct . | 4-OH-T . | Myeloid cytokine colonies, no. . | Lymphoid cytokine colonies, no. . | Colonies with no cytokines, no. . |
|---|---|---|---|---|
| MycER | + | 930 ± 50 | 57 ± 3 | 207 ± 18 |
| MycER | − | 390 ± 50 | 64 ± 6 | 41 ± 5 |
| GFP | + | 410 ± 40 | 34 ± 5 | 5 ± 1 |
| GFP | − | 440 ± 30 | 35 ± 3 | 8 ± 1 |
Bone marrow from mice that underwent transplantation with the indicated retroviruses was sorted for GFP and plated in methylcellulose media containing myeloid cytokines (SCF, IL-3, IL-6, Epo; 5 × 104 cells/well), lymphoid cytokines (IL-7; 4 × 105 cells/well), or no cytokines (4 × 105 cells/well). Cells were plated in triplicate in each experiment. Myeloid colony numbers were multiplied by 10 to normalize for number of cells plated. Colony numbers are ± SD. A representative of 4 independent experiments is shown.
4-OH-T indicates 4-hydroxy-tamoxifen.