Binding of anti-GP Ib monoclonal antibodies to GP Ibα mutants
. | AN51 . | MB45 . | AK2 . | Hip1 . | 6D1 . | AP1 . | SZ2 . |
|---|---|---|---|---|---|---|---|
| Wild-type | 93 ± 9 | 126 ± 15 | 93 ± 14 | 96 ± 10 | 124 ± 7 | 150 ± 12 | 88 ± 8 |
| His37Ala | 108 ± 14 | 147 ± 23 | 36 ± 2 | 13 ± 1 | 101 ± 16 | 103 ± 12 | 81 ± 6 |
| Glu40Ala | 125 ± 10 | 132 ± 12 | 34 ± 2 | 8 ± 0 | 114 ± 7 | 106 ± 5 | 101 ± 10 |
| Asp83Ala | 173 ± 11 | 134 ± 12 | 63 ± 2 | 79 ± 0 | 110 ± 10 | 106 ± 3 | 137 ± 8 |
| His86Ala | 234 ± 17 | 204 ± 12 | 28 ± 4 | 78 ± 5 | 152 ± 13 | 123 ± 9 | 174 ± 21 |
| Asp106Ala | 68 ± 19 | 87 ± 10 | 71 ± 1 | 71 ± 7 | 84 ± 6 | 88 ± 4 | 56 ± 5 |
| Glu128Ala | 186 ± 9 | 163 ± 16 | 28 ± 1 | 4 ± 0 | 160 ± 7 | 131 ± 8 | 158 ± 4 |
| Lys137Ala | 96 ± 19 | 106 ± 3 | 74 ± 3 | 81 ± 0 | 100 ± 10 | 122 ± 8 | 71 ± 9 |
| Lys152Ala | 128 ± 13 | 129 ± 15 | 58 ± 6 | 93 ± 12 | 117 ± 5 | 113 ± 11 | 113 ± 2 |
| Glu162Ala | 86 ± 19 | 111 ± 17 | 74 ± 2 | 86 ± 11 | 97 ± 9 | 110 ± 8 | 64 ± 5 |
| Asp175Ala | 199 ± 15 | 161 ± 8 | 25 ± 1 | 78 ± 6 | 135 ± 8 | 109 ± 4 | 147 ± 8 |
| Lys189Ala | 79 ± 24 | 96 ± 14 | 75 ± 1 | 72 ± 3 | 90 ± 7 | 95 ± 3 | 56 ± 7 |
. | AN51 . | MB45 . | AK2 . | Hip1 . | 6D1 . | AP1 . | SZ2 . |
|---|---|---|---|---|---|---|---|
| Wild-type | 93 ± 9 | 126 ± 15 | 93 ± 14 | 96 ± 10 | 124 ± 7 | 150 ± 12 | 88 ± 8 |
| His37Ala | 108 ± 14 | 147 ± 23 | 36 ± 2 | 13 ± 1 | 101 ± 16 | 103 ± 12 | 81 ± 6 |
| Glu40Ala | 125 ± 10 | 132 ± 12 | 34 ± 2 | 8 ± 0 | 114 ± 7 | 106 ± 5 | 101 ± 10 |
| Asp83Ala | 173 ± 11 | 134 ± 12 | 63 ± 2 | 79 ± 0 | 110 ± 10 | 106 ± 3 | 137 ± 8 |
| His86Ala | 234 ± 17 | 204 ± 12 | 28 ± 4 | 78 ± 5 | 152 ± 13 | 123 ± 9 | 174 ± 21 |
| Asp106Ala | 68 ± 19 | 87 ± 10 | 71 ± 1 | 71 ± 7 | 84 ± 6 | 88 ± 4 | 56 ± 5 |
| Glu128Ala | 186 ± 9 | 163 ± 16 | 28 ± 1 | 4 ± 0 | 160 ± 7 | 131 ± 8 | 158 ± 4 |
| Lys137Ala | 96 ± 19 | 106 ± 3 | 74 ± 3 | 81 ± 0 | 100 ± 10 | 122 ± 8 | 71 ± 9 |
| Lys152Ala | 128 ± 13 | 129 ± 15 | 58 ± 6 | 93 ± 12 | 117 ± 5 | 113 ± 11 | 113 ± 2 |
| Glu162Ala | 86 ± 19 | 111 ± 17 | 74 ± 2 | 86 ± 11 | 97 ± 9 | 110 ± 8 | 64 ± 5 |
| Asp175Ala | 199 ± 15 | 161 ± 8 | 25 ± 1 | 78 ± 6 | 135 ± 8 | 109 ± 4 | 147 ± 8 |
| Lys189Ala | 79 ± 24 | 96 ± 14 | 75 ± 1 | 72 ± 3 | 90 ± 7 | 95 ± 3 | 56 ± 7 |
Values are percentages of the values obtained with the WM23 antibody and represent the mean ± SEM.