A comparison of ADAMTS13 assay methods.
| Authors . | Substrate . | Denaturant . | Incubation Time . | Detection Method . | Indication of VWF Proteolysis . | Ref. . |
|---|---|---|---|---|---|---|
| Abbreviations: VWF, von Willebrand factor | ||||||
| Furlan et al | purified VWF | 1.5 mol/L urea | overnight | SDS-agarose gel electrophoresis, immunoblotting | decreased multimer size | 7 |
| Tsai | purified VWF | 0.15 mol/L guanidine HCl | 1 hour | SDS-polyacrylamide gel electrophoresis, immunoblotting | generation of dimer of 176 kDa fragment | 8 |
| Obert et al | recombinant VWF | 1.5 mol/L urea | overnight | two-site immuno- radiometric assay | decreased VWF antigen | 9 |
| Gerritsen et al | VWF in EDTA- treated and dialyzed plasma | 1.5 mol/L urea | 2 hour | residual collagen binding | decreased collagen binding | 10 |
| Böhm et al | purified VWF | 1.5 mol/L urea | overnight | residual ristocetin cofactor activity | decrease of ristocetin induced platelet aggregation | 11 |
| Kokame et al | recombinant VWF fragment, VWF73 | none | 20–60 min | SDS-polyacrylamide gel electrophoresis, immunoblotting | generation of proteolytic fragment | 13 |
| Whitelock et al | VWF A2 domain | none | 2 hour | enzyme linked immunosorbent assay | decrease of intact A2 domain | 14 |
| Zhou and Tsai | recombinant VWF fragment, VWF73 | none | 3 hour | enzyme immunoassay | decrease of VWF73 | 16 |
| Authors . | Substrate . | Denaturant . | Incubation Time . | Detection Method . | Indication of VWF Proteolysis . | Ref. . |
|---|---|---|---|---|---|---|
| Abbreviations: VWF, von Willebrand factor | ||||||
| Furlan et al | purified VWF | 1.5 mol/L urea | overnight | SDS-agarose gel electrophoresis, immunoblotting | decreased multimer size | 7 |
| Tsai | purified VWF | 0.15 mol/L guanidine HCl | 1 hour | SDS-polyacrylamide gel electrophoresis, immunoblotting | generation of dimer of 176 kDa fragment | 8 |
| Obert et al | recombinant VWF | 1.5 mol/L urea | overnight | two-site immuno- radiometric assay | decreased VWF antigen | 9 |
| Gerritsen et al | VWF in EDTA- treated and dialyzed plasma | 1.5 mol/L urea | 2 hour | residual collagen binding | decreased collagen binding | 10 |
| Böhm et al | purified VWF | 1.5 mol/L urea | overnight | residual ristocetin cofactor activity | decrease of ristocetin induced platelet aggregation | 11 |
| Kokame et al | recombinant VWF fragment, VWF73 | none | 20–60 min | SDS-polyacrylamide gel electrophoresis, immunoblotting | generation of proteolytic fragment | 13 |
| Whitelock et al | VWF A2 domain | none | 2 hour | enzyme linked immunosorbent assay | decrease of intact A2 domain | 14 |
| Zhou and Tsai | recombinant VWF fragment, VWF73 | none | 3 hour | enzyme immunoassay | decrease of VWF73 | 16 |