Ex vivo approaches to progenitor and stem cell expansion.
| Protocol . | Evidence for HSC Expansion . | Application to Clinical Trials . |
|---|---|---|
| Abbreviations: HSC, hematopoietic stem cells | ||
| CD34+ selected cells, high-dose cytokines | + | Limited trials, without best combinations due to cytokine availability. No nadir reduction. |
| Low-dose cytokines, continuous perfusion | ? | In trials, without best cytokine combinations due to cytokine availability. No nadir reduction. |
| Direct transcriptional activation of HSC cycling: HOXB4, telomerase, NF-Y | +++ | Future |
| Protocol . | Evidence for HSC Expansion . | Application to Clinical Trials . |
|---|---|---|
| Abbreviations: HSC, hematopoietic stem cells | ||
| CD34+ selected cells, high-dose cytokines | + | Limited trials, without best combinations due to cytokine availability. No nadir reduction. |
| Low-dose cytokines, continuous perfusion | ? | In trials, without best cytokine combinations due to cytokine availability. No nadir reduction. |
| Direct transcriptional activation of HSC cycling: HOXB4, telomerase, NF-Y | +++ | Future |