GO functional analysis of upregulated genes with associated SRF peaks
| Comparison . | Molecular function . | Cellular component . | Predicted regulators or transcription factors . |
|---|---|---|---|
| Untreated vs TPA+ (n = 172) | Cytoskeletal protein binding (5.295E-7) | Focal adhesion (6.179E-7) | SRF (3.223E-8) |
| ETS (2.335E-6) | |||
| Untreated vs MRTFAOETPA+ (n = 503) | Cytoskeletal protein binding (4.063E-14) | Focal adhesion (1.493E-17) | SRF (2.045E-14) |
| Actin binding (2.414E-13) | |||
| TPA+ vs MRTFAOETPA+ (n = 309) | Actin binding (1.157E-4) | Actin cytoskeleton (9.147E-7) | SRF (8.993E-14) |
| Actin filament binding (6.680E-3) | Focal adhesion (6.257E-6) |
| Comparison . | Molecular function . | Cellular component . | Predicted regulators or transcription factors . |
|---|---|---|---|
| Untreated vs TPA+ (n = 172) | Cytoskeletal protein binding (5.295E-7) | Focal adhesion (6.179E-7) | SRF (3.223E-8) |
| ETS (2.335E-6) | |||
| Untreated vs MRTFAOETPA+ (n = 503) | Cytoskeletal protein binding (4.063E-14) | Focal adhesion (1.493E-17) | SRF (2.045E-14) |
| Actin binding (2.414E-13) | |||
| TPA+ vs MRTFAOETPA+ (n = 309) | Actin binding (1.157E-4) | Actin cytoskeleton (9.147E-7) | SRF (8.993E-14) |
| Actin filament binding (6.680E-3) | Focal adhesion (6.257E-6) |
With MRTFAOE, the SRF/MRTFA regulatory axis takes precedence in HEL cell TPA-induced maturation. Binding site analysis of the genes, with an associated SRF peak, upregulated during TPA induction predicts both SRF and ETS as regulatory transcription factors. When comparing untreated and MRTFAOETPA+ groups, the actin cytoskeleton becomes the predominant molecular function, and SRF is the major transcription factor. When comparing TPA+ and MRTFAOETPA+ groups, actin cytoskeletal pathways become more significant, and SRF is also a more significant predicted transcription factor. Numbers in parentheses denote Bonferroni-corrected P values.