Characteristics of unexposed RBCs and dRBCs
| Sample . | Hb, g/dL . | MCHC, g/dL . | Diameter, µm . | MCV, µm3 . | Projected area, µm2 . | S/V, µm−1 . | Sphericity . | Relative rigidity . | Retention rate, % . | |
|---|---|---|---|---|---|---|---|---|---|---|
| Spleen . | Microsphilters . | |||||||||
| Untreated RBCs | 11.3 (1.3) | 34 (2) | 9.2 (0.003) | 92 (2) | 67 (0.01) | 0.73 (0.02) | 0.82 (0.01) | 1.0 (0.2) | 9 (5) | 0 (0) |
| Diamide-treated RBCs | ||||||||||
| 10-60 µM | 11.8 (1.9) | 34 (2) | 9.4 (0.04) | 91 (2) | 70 (0.4) | 0.77 (0.02) | 0.82 (0.001) | 1.3 (0.4) | 24 (13) | 2 (3) |
| 75-250 µM | 11.5 (1.6) | 34 (1) | 9.4 (0.1) | 91 (4) | 70 (2) | 0.77 (0.04) | 0.83 (0.01) | 4.1 (1.7) | 41 (5) | 19 (16) |
| 300-500 µM | 11.4 (1.3) | 33 (1) | 9.5 (0.1) | 95 (4) | 72 (2) | 0.76 (0.04) | 0.83 (0.02) | 17.4 (4.3) | 58 (5) | 13 (13) |
| Sample . | Hb, g/dL . | MCHC, g/dL . | Diameter, µm . | MCV, µm3 . | Projected area, µm2 . | S/V, µm−1 . | Sphericity . | Relative rigidity . | Retention rate, % . | |
|---|---|---|---|---|---|---|---|---|---|---|
| Spleen . | Microsphilters . | |||||||||
| Untreated RBCs | 11.3 (1.3) | 34 (2) | 9.2 (0.003) | 92 (2) | 67 (0.01) | 0.73 (0.02) | 0.82 (0.01) | 1.0 (0.2) | 9 (5) | 0 (0) |
| Diamide-treated RBCs | ||||||||||
| 10-60 µM | 11.8 (1.9) | 34 (2) | 9.4 (0.04) | 91 (2) | 70 (0.4) | 0.77 (0.02) | 0.82 (0.001) | 1.3 (0.4) | 24 (13) | 2 (3) |
| 75-250 µM | 11.5 (1.6) | 34 (1) | 9.4 (0.1) | 91 (4) | 70 (2) | 0.77 (0.04) | 0.83 (0.01) | 4.1 (1.7) | 41 (5) | 19 (16) |
| 300-500 µM | 11.4 (1.3) | 33 (1) | 9.5 (0.1) | 95 (4) | 72 (2) | 0.76 (0.04) | 0.83 (0.02) | 17.4 (4.3) | 58 (5) | 13 (13) |
Hemoglobin (Hb), MCHC, and mean cell volume (MCV) were measured with an ADVIA hematology system. Sphericity = aspect ratio. All data are mean (standard deviation).