Molecular mass, Rg, Dmax, and allosteric activation for ADAMTS13 internal deletions
| Variant . | Mass (kDa) . | Rg (Å), mean ± SD . | Dmax (Å), mean ± SD . | Enzymatic rate, pH 7.4 (min−1) (mean) . | Activation by MAbs (fold) (mean) . | Activation by D4 (fold) (mean) . |
|---|---|---|---|---|---|---|
| delT4L | 144 | 64 ± 3 | 217 ± 15 | 2.4 | 2.0 | 1.3 |
| delT4pL | 138 | 75 ± 5 | 268 ± 5 | 3.1 | 1.8 | 1.7 |
| delT7 | 141 | 76 ± 4 | 258 ± 14 | 2.7 | 2.6 | 1.0 |
| delT8 | 141 | 77 ± 4 | 267 ± 16 | 4.1 | 2.0 | 1.0 |
| delT8L | 141 | 75 ± 4 | 256 ± 18 | 3.6 | 2.6 | 1.0 |
| delT8pL | 135 | IM | IM | 4.3 | 2.0 | 1.0 |
| delT2-7 | 109 | 57 ± 2 | 189 ± 14 | 3.6 | 3.0 | 1.0 |
| delT2-8L | 103 | AiS | AiS | n/a | 1.4 | 1.0 |
| delT3-6 | 117 | 70 ± 3 | 244 ± 8 | 2.6 | 2.5 | 1.5 |
| pADAMTS13 | 116 | 56 ± 1 | 190 ± 10 | 0.2 | 1.0 | 4.0 |
| ADAMTS13 | 147 | 67 ± 2 | 229 ± 6 | 2.2 | 3.8 | 1.8 |
| Variant . | Mass (kDa) . | Rg (Å), mean ± SD . | Dmax (Å), mean ± SD . | Enzymatic rate, pH 7.4 (min−1) (mean) . | Activation by MAbs (fold) (mean) . | Activation by D4 (fold) (mean) . |
|---|---|---|---|---|---|---|
| delT4L | 144 | 64 ± 3 | 217 ± 15 | 2.4 | 2.0 | 1.3 |
| delT4pL | 138 | 75 ± 5 | 268 ± 5 | 3.1 | 1.8 | 1.7 |
| delT7 | 141 | 76 ± 4 | 258 ± 14 | 2.7 | 2.6 | 1.0 |
| delT8 | 141 | 77 ± 4 | 267 ± 16 | 4.1 | 2.0 | 1.0 |
| delT8L | 141 | 75 ± 4 | 256 ± 18 | 3.6 | 2.6 | 1.0 |
| delT8pL | 135 | IM | IM | 4.3 | 2.0 | 1.0 |
| delT2-7 | 109 | 57 ± 2 | 189 ± 14 | 3.6 | 3.0 | 1.0 |
| delT2-8L | 103 | AiS | AiS | n/a | 1.4 | 1.0 |
| delT3-6 | 117 | 70 ± 3 | 244 ± 8 | 2.6 | 2.5 | 1.5 |
| pADAMTS13 | 116 | 56 ± 1 | 190 ± 10 | 0.2 | 1.0 | 4.0 |
| ADAMTS13 | 147 | 67 ± 2 | 229 ± 6 | 2.2 | 3.8 | 1.8 |
Rg data were calculated using the program AutoRg, and Dmax data were calculated using the program DATGNOM. Standard error for enzymatic rates and activation is <10%.
AiS, aggregation in solution; IM, insufficient material for SAXS; n/a, not available.