Molecular mass, Rg, Dmax, and activity assay at pH 6.0 and pH 7.4 for ADAMTS13 truncation mutants
| Variant . | Mass (kDa) . | Rg (Å), mean ± SD . | Dmax (Å), mean ± SD . | Enzymatic rate, pH 6.0 (min−1) (mean) . | Enzymatic rate, pH 7.4 (min−1) (mean) . |
|---|---|---|---|---|---|
| MDTCS | 67 | 45 ± 1 | 148 ± 2 | 9.9 | 10.0 |
| MT2 | 74 | 48 ± 3 | 155 ± 6 | 9.2 | 10.2 |
| MT3 | 81 | 54 ± 5 | 180 ± 10 | 9.7 | 9.7 |
| MT4 | 87 | 59 ± 4 | 193 ± 16 | 7.8 | 9.8 |
| MT5 | 96 | 63 ± 3 | 210 ± 9 | 7.2 | 9.4 |
| MT6 | 103 | 67 ± 5 | 234 ± 15 | 8.9 | 9.3 |
| MT7 | 109 | 64 ± 2 | 210 ± 11 | 8.3 | 8.9 |
| MT8 | 115 | 63 ± 1 | 215 ± 9 | 3.5 | 4.0 |
| MT8L | 121 | 65 ± 3 | 206 ± 2 | 5.3 | 4.7 |
| ADAMTS13 | 147 | 67 ± 2 | 229 ± 6 | 8.0 | 2.2 |
| Variant . | Mass (kDa) . | Rg (Å), mean ± SD . | Dmax (Å), mean ± SD . | Enzymatic rate, pH 6.0 (min−1) (mean) . | Enzymatic rate, pH 7.4 (min−1) (mean) . |
|---|---|---|---|---|---|
| MDTCS | 67 | 45 ± 1 | 148 ± 2 | 9.9 | 10.0 |
| MT2 | 74 | 48 ± 3 | 155 ± 6 | 9.2 | 10.2 |
| MT3 | 81 | 54 ± 5 | 180 ± 10 | 9.7 | 9.7 |
| MT4 | 87 | 59 ± 4 | 193 ± 16 | 7.8 | 9.8 |
| MT5 | 96 | 63 ± 3 | 210 ± 9 | 7.2 | 9.4 |
| MT6 | 103 | 67 ± 5 | 234 ± 15 | 8.9 | 9.3 |
| MT7 | 109 | 64 ± 2 | 210 ± 11 | 8.3 | 8.9 |
| MT8 | 115 | 63 ± 1 | 215 ± 9 | 3.5 | 4.0 |
| MT8L | 121 | 65 ± 3 | 206 ± 2 | 5.3 | 4.7 |
| ADAMTS13 | 147 | 67 ± 2 | 229 ± 6 | 8.0 | 2.2 |
SAXS was performed with E225Q variants. Rg was calculated from Guinier plots, and Dmax was calculated from P(r) profiles. Standard error for all enzymatic rates is <10%.
SD, standard deviation.