HLA matching status of the patient in the clinical case and potential unrelated donors
| Subject . | Age (y) . | HLA-A* . | HLA-B* . | HLA-C* . | HLA-DRB1* . | HLA-DQB1* . | HLA-DPB1* . | TCE3† . | SNP proxy‡ . | HLA allele matching . |
|---|---|---|---|---|---|---|---|---|---|---|
| Patient | 60 | 03:01, 24:02 | 07:02, 38:01 | 07:02, 12:03 | 01:01, 13:01 | 05:01, 06:03 | 04:01, 04:02 | 3/3 | A/A | |
| Donor 1 | 42 | 03:01, 24:02 | 07:02, 38:01 | 07:02, 12:03 | 01:01, 13:01 | 05:01, 06:03 | 04:01, 04:02 | 3/3, NA | A/A, NA | 8/8 (12/12) |
| Donor 2 | 44 | 03:01, 24:02 | 07:02, 38:01 | 07:02, 12:03 | 01:01, 13:01 | 05:01, 06:03 | 02:01, 04:02 | 3/3, P | A/A, LR | 8/8 (11/12) |
| Donor 3 | 41 | 03:01, 24:02 | 07:02, 38:01 | 07:02, 12:03 | 01:01, 13:01 | 05:01, 06:03 | 02:01, 03:01 | 3/2, NP | A/G, NA | 8/8 (10/12) |
| Donor 4 | 28 | 02:01, 24:02 | 07:02, 38:01 | 07:02, 12:03 | 01:01, 13:01 | 05:01, 06:03 | 14:01, 09:01 | 2/1, NP | G/G, NA | 7/8 (9/12) |
| Subject . | Age (y) . | HLA-A* . | HLA-B* . | HLA-C* . | HLA-DRB1* . | HLA-DQB1* . | HLA-DPB1* . | TCE3† . | SNP proxy‡ . | HLA allele matching . |
|---|---|---|---|---|---|---|---|---|---|---|
| Patient | 60 | 03:01, 24:02 | 07:02, 38:01 | 07:02, 12:03 | 01:01, 13:01 | 05:01, 06:03 | 04:01, 04:02 | 3/3 | A/A | |
| Donor 1 | 42 | 03:01, 24:02 | 07:02, 38:01 | 07:02, 12:03 | 01:01, 13:01 | 05:01, 06:03 | 04:01, 04:02 | 3/3, NA | A/A, NA | 8/8 (12/12) |
| Donor 2 | 44 | 03:01, 24:02 | 07:02, 38:01 | 07:02, 12:03 | 01:01, 13:01 | 05:01, 06:03 | 02:01, 04:02 | 3/3, P | A/A, LR | 8/8 (11/12) |
| Donor 3 | 41 | 03:01, 24:02 | 07:02, 38:01 | 07:02, 12:03 | 01:01, 13:01 | 05:01, 06:03 | 02:01, 03:01 | 3/2, NP | A/G, NA | 8/8 (10/12) |
| Donor 4 | 28 | 02:01, 24:02 | 07:02, 38:01 | 07:02, 12:03 | 01:01, 13:01 | 05:01, 06:03 | 14:01, 09:01 | 2/1, NP | G/G, NA | 7/8 (9/12) |
Mismatched alleles in the donor are indicated in bold.
LR, low risk because donor 2 and the recipient differ only for a single HLA-DPB1 allele and the mismatched alleles both carry the low-expressing rs9277534-A variant; NA, not applicable because donor and recipient are identical for both HLA-DPB1 alleles (donor 1) or mismatched for both HLA-DPB1 alleles (donors 3 and 4); NP, nonpermissive because one (donor 3) or both (donor 4) of the mismatched HLA-DPB1 alleles in the donor (in bold) belong to a different TCE group from the patient’s; P, permissive because the mismatched HLA-DPB1 allele in the donor (in bold) belongs to the same TCE group as the patient’s; SNP, single-nucleotide polymorphism; TCE3, T-cell epitope 3-group model.
Functional HLA-DPB1 matching by TCE3.12 The numbers indicate to which of the 3 TCE groups (1, 2, or 3) the first (before the slash) and the second (after the slash) HLA-DPB1 allele in each individual belongs. Classification by TCE3 can be performed via a tool freely available online at https://www.ebi.ac.uk/ipd/imgt/hla/dpb.html.
Functional HLA-DPB1 matching by the SNP proxy.13 The letters A and G refer to the nucleotide carried at the rs9277534 SNP by the first (before the slash) and the second (after the slash) HLA-DPB1 allele in each individual.