Table 1.

Kd for lipid vesicle binding to indicated PKC C1 domains determined by intrinsic tryptophan fluorescence

Vesicle Lipid
C1 domainControl*DAG†HAGP‡HAG§
PKCα C1a 280 ± 65 5.52 ± 2.0 5.41 ± 1.6 13.4 ± 3.9 
PKCδ C1a 300 ± 86 14.6 ± 2.0 24.6 ± 5.7 20.5 ± 7.0 
PKCδ C1b 278 ± 7.5 5.00 ± 0 ND 22.0 ± 1.5 
Vesicle Lipid
C1 domainControl*DAG†HAGP‡HAG§
PKCα C1a 280 ± 65 5.52 ± 2.0 5.41 ± 1.6 13.4 ± 3.9 
PKCδ C1a 300 ± 86 14.6 ± 2.0 24.6 ± 5.7 20.5 ± 7.0 
PKCδ C1b 278 ± 7.5 5.00 ± 0 ND 22.0 ± 1.5 

All Kd are in units of nanomolar.

ND, not determined.

*

Control vesicles contained only DOPC and DOPS.

†

Vesicles contained 20 mol% DAG in addition to DOPC and DOPS.

‡

Vesicles contained 20 mol% HAGP in addition to DOPC and DOPS.

§

Vesicles contained 20 mol% HAG in addition to DOPC and DOPS.

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