Annexin V binding by CLL cells
| . | NA . | + IL-4 . | + PF . | +IL-4 + PF . |
|---|---|---|---|---|
| CLL21 | ||||
| No flu | 0.9 | 0.8 | 1.0 | 1.4 |
| 1μM flu | 12.4 | 2 | 16.7 | 12.7 |
| 2μM flu | 23.0 | 3.5 | 24.6 | 27.5 |
| CLL23 | ||||
| No flu | 0.1 | 3.3 | 0.3 | 1.4 |
| 1μM flu | 28.4 | 9.4 | 36.8 | 25.8 |
| CLL16 | ||||
| No flu | 2.3 | 1.3 | 6.0 | 1.6 |
| 0.5μM flu | 3.4 | 1 | 7.6 | 4.1 |
| 1μM flu | 6.3 | 2.3 | 11.2 | 5.9 |
| 2μM flu | 16.0 | 4.6 | 17.4 | 12.5 |
| CLL13 | ||||
| No flu | 0.7 | 1.2 | 7.0 | 4.8 |
| 2μM flu | 26.8 | 14.0 | 39.8 | 25.3 |
| . | NA . | + IL-4 . | + PF . | +IL-4 + PF . |
|---|---|---|---|---|
| CLL21 | ||||
| No flu | 0.9 | 0.8 | 1.0 | 1.4 |
| 1μM flu | 12.4 | 2 | 16.7 | 12.7 |
| 2μM flu | 23.0 | 3.5 | 24.6 | 27.5 |
| CLL23 | ||||
| No flu | 0.1 | 3.3 | 0.3 | 1.4 |
| 1μM flu | 28.4 | 9.4 | 36.8 | 25.8 |
| CLL16 | ||||
| No flu | 2.3 | 1.3 | 6.0 | 1.6 |
| 0.5μM flu | 3.4 | 1 | 7.6 | 4.1 |
| 1μM flu | 6.3 | 2.3 | 11.2 | 5.9 |
| 2μM flu | 16.0 | 4.6 | 17.4 | 12.5 |
| CLL13 | ||||
| No flu | 0.7 | 1.2 | 7.0 | 4.8 |
| 2μM flu | 26.8 | 14.0 | 39.8 | 25.3 |
The percentages of apoptotic annexin V–positive/propidium iodide–negative cells were determined by FACscan analysis, as shown in Figure 4. IL-4 and PF were added at 10 ng/mL and 1μM, respectively.
NA indicates no addition; and PF, PF-956980.