Modulation of ADIR relative gene expression in PBMCs
| Incubation time, h . | ADIR . | PBGD . | ||||
|---|---|---|---|---|---|---|
| Control . | IFN . | PHA . | Control . | IFN . | PHA . | |
| 0 | 9 ± 3 | 9 ± 3 | 9 ± 3 | 5 ± 1 | 5 ± 1 | 5 ± 1 |
| 24 | 24 ± 4 | 19 ± 6 | 48 ± 22 | 5 ± 2 | 3 ± 1 | 60 ± 53 |
| 48 | 36 ± 17 | 23 ± 10 | 238 ± 91 | 8 ± 2 | 5 ± 2 | 178 ± 153 |
| Incubation time, h . | ADIR . | PBGD . | ||||
|---|---|---|---|---|---|---|
| Control . | IFN . | PHA . | Control . | IFN . | PHA . | |
| 0 | 9 ± 3 | 9 ± 3 | 9 ± 3 | 5 ± 1 | 5 ± 1 | 5 ± 1 |
| 24 | 24 ± 4 | 19 ± 6 | 48 ± 22 | 5 ± 2 | 3 ± 1 | 60 ± 53 |
| 48 | 36 ± 17 | 23 ± 10 | 238 ± 91 | 8 ± 2 | 5 ± 2 | 178 ± 153 |
PBMCs were incubated for the indicated times in the absence or presence of 1000 IU/mL INF-α or 0.8 μg/mL PHA. Prior to mRNA isolation and cDNA synthesis, 1% mouse spleen cells were added to each sample. Data were normalized to murine GAPDH expression. Expression of EBV-LCLs was set to 100.