Table 1

Myeloid progenitor populations in WT and Mx1-Cre, KrasGI2D mice

Population and organWTMx1-Cre, KrasGI2DRatio*
KLS    
    Marrow† 24 ± 13 14 ± 5.0  
    Spleen 7.4 ± 5.8 1300 ± 36  
    Total‡ 127 1400 11 
CMP    
    Marrow 210 ± 45 34 ± 10  
    Spleen 25 ± 9.4 1100 ± 250  
    Total 1100 1300 1.2 
GMP    
    Marrow 420 ± 76 220 ± 92  
    Spleen 7.8 ± 3.9 5200 ± 1400  
    Total 2100 6300 3.0 
MEP    
    Marrow 210 ± 48 52 ± 21  
    Spleen 65 ± 40 4900 ± 1600  
    Total 1100 5100 4.6 
Population and organWTMx1-Cre, KrasGI2DRatio*
KLS    
    Marrow† 24 ± 13 14 ± 5.0  
    Spleen 7.4 ± 5.8 1300 ± 36  
    Total‡ 127 1400 11 
CMP    
    Marrow 210 ± 45 34 ± 10  
    Spleen 25 ± 9.4 1100 ± 250  
    Total 1100 1300 1.2 
GMP    
    Marrow 420 ± 76 220 ± 92  
    Spleen 7.8 ± 3.9 5200 ± 1400  
    Total 2100 6300 3.0 
MEP    
    Marrow 210 ± 48 52 ± 21  
    Spleen 65 ± 40 4900 ± 1600  
    Total 1100 5100 4.6 

Stem and myeloid progenitor populations were enumerated by flow cytometry. All numbers indicate absolute numbers, in thousands, of cells recovered (n = 3; mean ± SEM).

*

Ratio of Mx1-Cre, KrasGI2D to WT.

†

Per 2 tibiae and 2 femora.

‡

The total number of cells in each population was estimated based on the assumption that tibiae and femora comprise 20% of total marrow space.27 

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