FITC-fMLP bound to the fMLP receptors, at various concentrations of FITC-fMLP
FITC-fMLP concentration, nM . | FITC-fMLP bound to the fMLP receptors, arbitrary fluorescence units . | |
---|---|---|
WT . | PTEN−/− . | |
3 | 3.88 | 3.98 |
6 | 5.27 | 5.08 |
12 | 5.82 | 6.96 |
FITC-fMLP concentration, nM . | FITC-fMLP bound to the fMLP receptors, arbitrary fluorescence units . | |
---|---|---|
WT . | PTEN−/− . | |
3 | 3.88 | 3.98 |
6 | 5.27 | 5.08 |
12 | 5.82 | 6.96 |
Specific binding was determined by subtracting geometric mean fluorescence intensity in the presence of 10 μM unlabeled fMLP from the geometric mean fluorescence intensity in the absence of 10 μM unlabeled fMLP. Data shown are representative from one of multiple experiments with similar results.