Genes modulated by KLF4 activation in cHL cell lines
| Official symbol . | Full name . | Fold of induction . | |
|---|---|---|---|
| KMH2 . | L428 . | ||
| Up-regulated | |||
| APAF1 | Apoptotic protease activating factor | 2.84 ± 0.15 | 2.41 ± 0.21 |
| BAK1 | BCL2-antagonist/killer 1 | 13.18 ± 0.77 | 23.61 ± 7.26 |
| BCL10 | B-cell CLL/lymphoma 10 | 4.54 ± 1.07 | 3.53 ± 0.74 |
| CASP4 | Caspase 4 | 9.40 ± 1.21 | 41.40 ± 3.49 |
| CASP5 | Caspase 5 | 9.71 ± 0.46 | 17.38 ± 0.48 |
| CASP7 | Caspase 7 | 3.75 ± 1.25 | 6.18 ± 0.22 |
| c-FLIP | CASP8 and FADD-like apoptosis regulator | 2.59 ± 0.08 | 2.28 ± 0.18 |
| DIABLO | Diablo homolog (Drosophila) | 3.17 ± 0.94 | 2.16 ± 0.48 |
| GADD45A | Growth arrest and DNA-damage-inducible, α | 6.85 ± 0.54 | 4.18 ± 2.31 |
| TNFRSF10A | Tumor necrosis factor receptor superfamily, member 10a | 4.13 ± 0.23 | 3.09 ± 0.43 |
| TNFRSF10C | Tumor necrosis factor receptor superfamily, member 10c | 3.40 ± 1.19 | 4.40 ± 0.45 |
| Down-regulated | |||
| RIPK2 | Receptor-interacting serine-threonine kinase 2 | 0.02 ± 0.01 | 0.36 ± 0.21 |
| TNFRSF9 | Tumor necrosis factor receptor superfamily, member 9 | 0.10 ± 0.06 | 0.41 ± 0.28 |
| DFFA | DNA fragmentation factor, 45 kDa, α polypeptide | 0.20 ± 0.08 | 0.39 ± 0.16 |
| Official symbol . | Full name . | Fold of induction . | |
|---|---|---|---|
| KMH2 . | L428 . | ||
| Up-regulated | |||
| APAF1 | Apoptotic protease activating factor | 2.84 ± 0.15 | 2.41 ± 0.21 |
| BAK1 | BCL2-antagonist/killer 1 | 13.18 ± 0.77 | 23.61 ± 7.26 |
| BCL10 | B-cell CLL/lymphoma 10 | 4.54 ± 1.07 | 3.53 ± 0.74 |
| CASP4 | Caspase 4 | 9.40 ± 1.21 | 41.40 ± 3.49 |
| CASP5 | Caspase 5 | 9.71 ± 0.46 | 17.38 ± 0.48 |
| CASP7 | Caspase 7 | 3.75 ± 1.25 | 6.18 ± 0.22 |
| c-FLIP | CASP8 and FADD-like apoptosis regulator | 2.59 ± 0.08 | 2.28 ± 0.18 |
| DIABLO | Diablo homolog (Drosophila) | 3.17 ± 0.94 | 2.16 ± 0.48 |
| GADD45A | Growth arrest and DNA-damage-inducible, α | 6.85 ± 0.54 | 4.18 ± 2.31 |
| TNFRSF10A | Tumor necrosis factor receptor superfamily, member 10a | 4.13 ± 0.23 | 3.09 ± 0.43 |
| TNFRSF10C | Tumor necrosis factor receptor superfamily, member 10c | 3.40 ± 1.19 | 4.40 ± 0.45 |
| Down-regulated | |||
| RIPK2 | Receptor-interacting serine-threonine kinase 2 | 0.02 ± 0.01 | 0.36 ± 0.21 |
| TNFRSF9 | Tumor necrosis factor receptor superfamily, member 9 | 0.10 ± 0.06 | 0.41 ± 0.28 |
| DFFA | DNA fragmentation factor, 45 kDa, α polypeptide | 0.20 ± 0.08 | 0.39 ± 0.16 |
A total of 2 × 106 KMH2-KLF4-ER and L428-KLF4-ER cells were seeded in 10 mL of complete culture medium and treated with 200nM of 4-OHT at the same day. At 24 hours (L428-KLF4-ER) or 48 hours later (KM-H2), cells were harvested, and expression of genes involved in apoptosis was analyzed using the human apoptosis primer set. Only genes whose expression was changed more than 2-fold in both cell lines are shown. The data are shown as fold modulation ± SD of 3 independent experiments.