Primers and probes used in quantitative RT-PCR
| GeneBank ID . | Gene . | . |
|---|---|---|
| 1) 5′-6(FAM)-PROBE-(TAMRA)-3′ | ||
| 2) 5′-PRIMER 1–3′ | ||
| 3) 5′-PRIMER 2–3′ | ||
| NM_002971 | SATB1 | 1) 5′-AACGAGCAGGAATCTCCCAGGCG-3′ |
| 2) 5′-ACCAGTGGGTACGCGATGA-3′ | ||
| 3) 5′-TGTTAAAAGCCACACGTGCAA-3′ | ||
| NM_003153 | STAT6 | 1) 5′-CAGGACACCATCAAACCACTGCCAAA-3′ |
| 2) 5′-TGGGCCGTGGCTTCAC-3′ | ||
| 3) 5′-CCGGAGACAGCGTTTGGT-3′ | ||
| NM_001002295 | GATA3 | 1) 5′-TGCCGGAGGAGGTGGATGTGCT-3′ |
| 2) 5′-GGACGCGGCGCAGTAC-3′ | ||
| 3) 5′-TGCCTTGACCGTCGATGTTA-3′ | ||
| NM_000879 | IL-5 | 1) 5′-TTGACTCTCCAGTGTGCCTATTCCCTGAA-3′ |
| 2) 5′-AAATCACCAACTGTGCACTGAA-3′ | ||
| 3) 5′-CGTCAATGTATTTCTTTATTAAGGACAA-3′ | ||
| NM_001402 | EF1α | 1) 5′-AGCGCCGGCTATGCCCCTG-3′ |
| 2) 5′-CTGAACCATCCAGGCCAAAT-3′ | ||
| 3) 5′-GCCGTGTGGCAATCCAAT-3′ |
| GeneBank ID . | Gene . | . |
|---|---|---|
| 1) 5′-6(FAM)-PROBE-(TAMRA)-3′ | ||
| 2) 5′-PRIMER 1–3′ | ||
| 3) 5′-PRIMER 2–3′ | ||
| NM_002971 | SATB1 | 1) 5′-AACGAGCAGGAATCTCCCAGGCG-3′ |
| 2) 5′-ACCAGTGGGTACGCGATGA-3′ | ||
| 3) 5′-TGTTAAAAGCCACACGTGCAA-3′ | ||
| NM_003153 | STAT6 | 1) 5′-CAGGACACCATCAAACCACTGCCAAA-3′ |
| 2) 5′-TGGGCCGTGGCTTCAC-3′ | ||
| 3) 5′-CCGGAGACAGCGTTTGGT-3′ | ||
| NM_001002295 | GATA3 | 1) 5′-TGCCGGAGGAGGTGGATGTGCT-3′ |
| 2) 5′-GGACGCGGCGCAGTAC-3′ | ||
| 3) 5′-TGCCTTGACCGTCGATGTTA-3′ | ||
| NM_000879 | IL-5 | 1) 5′-TTGACTCTCCAGTGTGCCTATTCCCTGAA-3′ |
| 2) 5′-AAATCACCAACTGTGCACTGAA-3′ | ||
| 3) 5′-CGTCAATGTATTTCTTTATTAAGGACAA-3′ | ||
| NM_001402 | EF1α | 1) 5′-AGCGCCGGCTATGCCCCTG-3′ |
| 2) 5′-CTGAACCATCCAGGCCAAAT-3′ | ||
| 3) 5′-GCCGTGTGGCAATCCAAT-3′ |
RT indicates reverse-transcribed polymerase chain reaction.