FACS analysis of cultures established from “fraction B” pro-B cells from wild-type (WT) and TAC4−/− mice cultured in the presence of IL-7 for 4 days
| Population . | B-cell stage . | Fraction . | WT . | TAC4−/− . | P . |
|---|---|---|---|---|---|
| Total cell number | 8.4 ± 0.1 × 105 | 1.2 ± 0.1 × 106 | .0108* | ||
| B220+CD117+ | unc → pro-B | A → C | 5.2 ± 0.6 × 105 | 7.7 ± 0.5 × 105 | .0243* |
| B220+CD117− | pre-B → mat | C′ → mat | 2.8 ± 0.2 × 105 | 3.2 ± 0.2 × 105 | .0926 |
| CD2−CD117+ | ELP → pro-B | ELP → C | 3.6 ± 0.6 × 105 | 5.4 ± 0.4 × 105 | .0434* |
| CD2−CD117− | pre-B | C′ | 2.7 ± 1.3 × 105 | 1.8 ± 0.3 × 105 | .5888 |
| CD2+CD117− | pre-B → mat | D → mat | 2.7 ± 0.4 × 105 | 3.5 ± 0.3 × 105 | .1208 |
| CD19+CD2−IgM− | pro-B → pre-B | B → C′ | 5.0 ± 0.7 × 105 | 7.1 ± 0.4 × 105 | .0498* |
| CD19+CD2+IgM− | pre-B | D | 2.5 ± 0.4 × 105 | 2.7 ± 0.3 × 105 | .5110 |
| CD19+CD2+IgM+ | imm → mat | E → mat | 4.7 ± 0.6 × 104 | 8.8 ± 1.0 × 104 | .0020** |
| CD19+CD2−IL7Rα+ | pro-B → pre-B | B → C′ | 5.3 ± 0.7 × 105 | 7.6 ± 0.6 × 105 | .0418* |
| CD19+CD2+IL7Rα+ | pre-B → imm | D,E | 2.2 ± 0.3 × 105 | 2.8 ± 0.3 × 105 | .1021 |
| CD19+CD2+IL7Rα− | imm → mat | imm → mat | 3.3 ± 0.7 × 104 | 4.0 ± 0.7 × 104 | .2405 |
| CD19+IgM−IgD− | pro-B → pre-B | B → D | 7.3 ± 0.7 × 105 | 9.9 ± 0.5 × 105 | .0245* |
| CD19+IgM+IgD− | imm | E | 5.7 ± 1.0 × 104 | 8.8 ± 1.0 × 104 | .0012** |
| CD19+IgM+IgD+ | imm → mat | imm → mat | 1.3 ± 0.2 × 104 | 2.4 ± 0.2 × 104 | .0094** |
| Population . | B-cell stage . | Fraction . | WT . | TAC4−/− . | P . |
|---|---|---|---|---|---|
| Total cell number | 8.4 ± 0.1 × 105 | 1.2 ± 0.1 × 106 | .0108* | ||
| B220+CD117+ | unc → pro-B | A → C | 5.2 ± 0.6 × 105 | 7.7 ± 0.5 × 105 | .0243* |
| B220+CD117− | pre-B → mat | C′ → mat | 2.8 ± 0.2 × 105 | 3.2 ± 0.2 × 105 | .0926 |
| CD2−CD117+ | ELP → pro-B | ELP → C | 3.6 ± 0.6 × 105 | 5.4 ± 0.4 × 105 | .0434* |
| CD2−CD117− | pre-B | C′ | 2.7 ± 1.3 × 105 | 1.8 ± 0.3 × 105 | .5888 |
| CD2+CD117− | pre-B → mat | D → mat | 2.7 ± 0.4 × 105 | 3.5 ± 0.3 × 105 | .1208 |
| CD19+CD2−IgM− | pro-B → pre-B | B → C′ | 5.0 ± 0.7 × 105 | 7.1 ± 0.4 × 105 | .0498* |
| CD19+CD2+IgM− | pre-B | D | 2.5 ± 0.4 × 105 | 2.7 ± 0.3 × 105 | .5110 |
| CD19+CD2+IgM+ | imm → mat | E → mat | 4.7 ± 0.6 × 104 | 8.8 ± 1.0 × 104 | .0020** |
| CD19+CD2−IL7Rα+ | pro-B → pre-B | B → C′ | 5.3 ± 0.7 × 105 | 7.6 ± 0.6 × 105 | .0418* |
| CD19+CD2+IL7Rα+ | pre-B → imm | D,E | 2.2 ± 0.3 × 105 | 2.8 ± 0.3 × 105 | .1021 |
| CD19+CD2+IL7Rα− | imm → mat | imm → mat | 3.3 ± 0.7 × 104 | 4.0 ± 0.7 × 104 | .2405 |
| CD19+IgM−IgD− | pro-B → pre-B | B → D | 7.3 ± 0.7 × 105 | 9.9 ± 0.5 × 105 | .0245* |
| CD19+IgM+IgD− | imm | E | 5.7 ± 1.0 × 104 | 8.8 ± 1.0 × 104 | .0012** |
| CD19+IgM+IgD+ | imm → mat | imm → mat | 1.3 ± 0.2 × 104 | 2.4 ± 0.2 × 104 | .0094** |
Columns WT and TAC4−/− show absolute cell numbers (mean ± SE, n = 10). Surface markers used for FACS analysis and the developmental stage of B-cell populations are indicated: ELP (early lymphoid progenitor), fractions A (uncommitted [unc]), B and C (pro-B), C′ and D (pre-B), E (immature [imm] and mature [mat]). P values were calculated from absolute cell numbers using paired 2-tailed t tests.
Significance (P < .05).
Significance (P < .005).