Table 2 FACS analysis of bone marrow... | American Society of Hematology

Table 2

FACS analysis of bone marrow cells from WT and TAC4^−/− mice cultured in the presence of IL-7 for 4 days

Population	B-cell stage	Fraction	WT	TAC4^−/−	P
Total cell number			5.6 ± 0.9 × 10⁶	8.2 ± 1.2 × 10⁶	.0647
B220⁺CD117⁺	Unc → pro-B	A → C	1.7 ± 0.4 × 10⁶	3.4 ± 0.7 × 10⁶	.0216*
B220⁺CD117⁻	pre-B → mat	C′ → mat	2.5 ± 0.4 × 10⁶	2.8 ± 0.5 × 10⁶	.5899
CD2⁻CD117⁺	ELP → pro-B	ELP → C	1.1 ± 0.3 × 10⁶	2.4 ± 0.5 × 10⁶	.0127*
CD2⁻CD117⁻	pre-B	C′	0.7 ± 0.2 × 10⁶	1.1 ± 0.2 × 10⁶	.0729
CD2⁺CD117⁻	pre-B → mat	D → mat	2.6 ± 0.5 × 10⁶	2.9 ± 0.6 × 10⁶	.6453
CD19⁺CD2⁻IgM⁻	pro-B → pre-B	B → C′	1.7 ± 0.4 × 10⁶	3.4 ± 0.6 × 10⁶	.0124*
CD19⁺CD2⁺IgM⁻	pre-B	D	1.9 ± 0.3 × 10⁶	2.0 ± 0.4 × 10⁶	.8244
CD19⁺CD2⁺IgM⁺	imm → mat	E → mat	0.7 ± 0.1 × 10⁶	1.1 ± 0.2 × 10⁶	.0969
CD19⁺CD2⁻IL7Rα⁺	pro-B → pre-B	B → C′	1.8 ± 0.4 × 10⁶	3.6 ± 0.6 × 10⁶	.0134*
CD19⁺CD2⁺IL7Rα⁺	pre-B → imm	D,E	1.7 ± 0.3 × 10⁶	1.9 ± 0.4 × 10⁶	.6064
CD19⁺CD2⁺IL7Rα⁻	imm → mat	imm → mat	0.7 ± 0.2 × 10⁶	0.7 ± 0.2 × 10⁶	.7970
CD19⁺IgM⁻IgD⁻	pro-B → pre-B	B → D	3.3 ± 0.6 × 10⁶	5.3 ± 0.8 × 10⁶	.0495*
CD19⁺IgM⁺IgD⁻	imm	E	0.7 ± 0.1 × 10⁶	0.9 ± 0.2 × 10⁶	.2613
CD19⁺IgM⁺IgD⁺	imm → mat	imm → mat	0.3 ± 0.1 × 10⁶	0.4 ± 0.1 × 10⁶	.0718

Population	B-cell stage	Fraction	WT	TAC4^−/−	P
Total cell number			5.6 ± 0.9 × 10⁶	8.2 ± 1.2 × 10⁶	.0647
B220⁺CD117⁺	Unc → pro-B	A → C	1.7 ± 0.4 × 10⁶	3.4 ± 0.7 × 10⁶	.0216*
B220⁺CD117⁻	pre-B → mat	C′ → mat	2.5 ± 0.4 × 10⁶	2.8 ± 0.5 × 10⁶	.5899
CD2⁻CD117⁺	ELP → pro-B	ELP → C	1.1 ± 0.3 × 10⁶	2.4 ± 0.5 × 10⁶	.0127*
CD2⁻CD117⁻	pre-B	C′	0.7 ± 0.2 × 10⁶	1.1 ± 0.2 × 10⁶	.0729
CD2⁺CD117⁻	pre-B → mat	D → mat	2.6 ± 0.5 × 10⁶	2.9 ± 0.6 × 10⁶	.6453
CD19⁺CD2⁻IgM⁻	pro-B → pre-B	B → C′	1.7 ± 0.4 × 10⁶	3.4 ± 0.6 × 10⁶	.0124*
CD19⁺CD2⁺IgM⁻	pre-B	D	1.9 ± 0.3 × 10⁶	2.0 ± 0.4 × 10⁶	.8244
CD19⁺CD2⁺IgM⁺	imm → mat	E → mat	0.7 ± 0.1 × 10⁶	1.1 ± 0.2 × 10⁶	.0969
CD19⁺CD2⁻IL7Rα⁺	pro-B → pre-B	B → C′	1.8 ± 0.4 × 10⁶	3.6 ± 0.6 × 10⁶	.0134*
CD19⁺CD2⁺IL7Rα⁺	pre-B → imm	D,E	1.7 ± 0.3 × 10⁶	1.9 ± 0.4 × 10⁶	.6064
CD19⁺CD2⁺IL7Rα⁻	imm → mat	imm → mat	0.7 ± 0.2 × 10⁶	0.7 ± 0.2 × 10⁶	.7970
CD19⁺IgM⁻IgD⁻	pro-B → pre-B	B → D	3.3 ± 0.6 × 10⁶	5.3 ± 0.8 × 10⁶	.0495*
CD19⁺IgM⁺IgD⁻	imm	E	0.7 ± 0.1 × 10⁶	0.9 ± 0.2 × 10⁶	.2613
CD19⁺IgM⁺IgD⁺	imm → mat	imm → mat	0.3 ± 0.1 × 10⁶	0.4 ± 0.1 × 10⁶	.0718

Columns WT and TAC4^−/− show absolute cell numbers (mean ± SE, n = 10). Surface markers used for FACS analysis and the developmental stage of B-cell populations are indicated: ELP (early lymphoid progenitor), fractions A (uncommitted [unc]), B and C (pro-B), C′ and D (pre-B), E (immature [imm] and mature [mat]). P values were calculated from absolute cell numbers using paired 2-tailed t tests.

Significance (P < .05).

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