Table 1

Anti-CD19 CAR-transduced T cells produced IFN-γ specifically in response to CD19

Effector T cells*CD19+ targets
CD19 targets
Effectors alone
38c13CD19-K562SplenocytesSol8CCL12NGFR-K562
1D3–28Z.1-3 286 480 234 252 16 378 1224 969 1531 1126 
1D3–28Z 294 150 297 100 18 085 5892 4480 6055 6267 
Not transduced 211 123 292 178 129 124 122 
Effector T cells*CD19+ targets
CD19 targets
Effectors alone
38c13CD19-K562SplenocytesSol8CCL12NGFR-K562
1D3–28Z.1-3 286 480 234 252 16 378 1224 969 1531 1126 
1D3–28Z 294 150 297 100 18 085 5892 4480 6055 6267 
Not transduced 211 123 292 178 129 124 122 

Values are the means of duplicate wells. The units are in picograms per milliliter of IFN-γ. All target cells cultured alone produced undetectable levels of IFN-γ.

*

Effector T cells were transduced with the indicated CAR as described in Figure 1C. Cultures containing the indicated effector cells and target cells were set up 1 day after transductions were completed.

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